Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0043167 (
pertussis
)
19,595
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We attempted to determine natural agonists of
GPR39
in fetal bovine serum (FBS). FBS was conditioned to extract peptides and fractionated by two types of HPLC. The activity of each fraction was monitored by intracellular calcium mobilization. Then the purified active ingredient was analyzed by inductively coupled plasma mass spectrometry. In this fashion, Zn2+ ion was identified as an agonist of
GPR39
, though no peptidergic molecules were found. The calcium-mobilizing activity of Zn2+ was not abolished by
pertussis
toxin but was by a phospholipase C (PLC) inhibitor, U73122, indicating that the activity of
GPR39
is mediated through the Gqalpha -PLC pathway. In addition, Zn2+ also activated mouse and rat
GPR39
, showing that the function of
GPR39
as a Zn2+ receptor is conserved across species. This study is the first exploration of
GPR39
agonists in FBS and indicates that
GPR39
functions as a Gq-coupled Zn2+-sensing receptor.
...
PMID:Isolation of Zn2+ as an endogenous agonist of GPR39 from fetal bovine serum. 1788 20
