Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0042961 (volvulus)
 4,305 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Anticoagulant activity was determined in salivary gland extracts from four species of black flies, i.e., Simulium vittatum Zetterstedt, Simulium argus Williston, Simulium metallicum Bellardi, and Simulium ochraceum Walker. Inhibition of coagulation factor Xa occurred among all four, whereas thrombin inhibition was detected in S. argus and S. vittatum only. Both bovine and human alpha-thrombins were inhibited with the highest activity occurring with S. argus salivary gland extracts. Factor Xa inhibition was highest in S. ochraceum, an anthropophilic species and vector of Onchocerca volvulus, and lowest in S. vittatum, a primiparous autogenous species that is also zoophilic. Total soluble salivary gland extract protein also varied among the four species with the highest concentration measured in S. ochraceum and the lowest in S. vittatum. A positive correlation was observed between the amount of soluble protein and percentage of inhibition of factor Xa for the four species.
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PMID:Anticoagulant activity in salivary gland extracts of black flies (Diptera: Simuliidae). 781 6

An expression vector, pOVEX, has been designed and constructed, combining the advantages of the expression vectors pGEX-3X and pJC2o. The pOVEX vector produces a fusion protein with the 24 kD Onchocerca volvulus glutathione S-transferase (OvGST2) which is easy to purify in one step from bacterial extracts under non-denaturing conditions using glutathione-sepharose chromatography. High yields of fusion protein were produced from this T7 RNA polymerase-dependent expression vector, which were then cleaved by digestion with the factor Xa protease to separate the OVGST2 polypeptide from the expressed protein of interest. This vector will be particularly useful to O. volvulus investigators for the production of O. volvulus antigens for the analyses of host humoral and cellular responses to these proteins and for immunization studies.
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PMID:pOVEX vector: prokaryotic expression and purification of onchocerciasis vaccine candidate antigens as fusion proteins with the 24 kD Onchocerca volvulus glutathione S-transferase. 927 Jul 37