Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0042109 (urticaria)
 6,569 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

C1q, a subcomponent of the first complement component, of a 60-year-old female patient with hypocomplementemic vasculitis-urticaria syndrome (HVUS) was characterized. The C1q-precipitin activity (C1q-p) could not be detected by a routine method with 0.6% agarose in 10 mM Na-phosphate buffer containing 10 mM EDTA (pH 7.2). Hemolytic activity of her serum complement (CH50) and levels of C1 and C4 were significantly reduced at the exacerbation stage, but levels of other complement components were almost within the normal range throughout her clinical course. The specific activity of C1q at her exacerbation stage was significantly low, and its elution position on Sephacryl S-300 column was spread toward the low molecular weight in comparison with that of normal plasma. Molecular weights of the delayed fraction of C1q were estimated to be approximately 300,000 on the Sephacryl and 440,000 by the polyacrylamide gel electrophoresis (PAGE) containing sodium dodecyl sulfate (SDS) followed by immunoblotting, respectively. On reduction of her plasma, two bands with molecular weights equivalent to those of B and C chains of the normal C1q in an approximate molar ratio of 2:1 were immunostained. Plasma at her exacerbation stage showed only one precipitation line against anti-human C1q-antiserum which was completely fused with that formed between purified normal human C1q and the same antiserum. The probable structural change of the hypofunctional C1q in the case of this HVUS is discussed.
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PMID:Characterization of C1q found in a patient with hypocomplementemic vasculitis-urticaria syndrome. 250 5

Although nickel-based (Ni-Cr and Ni-Cr-Be) alloy prothesis is widely used in orthodontics, its potential biologic hazards, hypersensitivity in particular, are still uncertain as yet. And only a few studies in vivo have considered the biocompatibility. However, several case reports show adverse effects of immunologic alterations, such as urticaria, respiratory disease, nickel contact dermatitis, microscopic hematuria and proteinuria, and even exacerbated to hepatocyte injury and renal injury. So nickel-based alloy used in dental restorations may be a potential cause for immune-mediated hypersensitivity. The metal surface would occur electrochemical corrosion as metal edge of porcelain-fused-to-nichrome crown exposed to oral cavity rich in electrolytes after restoration, and metal ion would release to oral cavity then come into contact with cells and tissues in the immediate environment, or be distributed throughout the body, mainly to the intestine canal. Once these ions are not biocompatible, the human system may be injured (toxicity and risk of sensitization) if they are absorbed in sufficient quantity. Thus, it is necessary to determine the long-term biocompatibility properties of nickel-based alloy, reduce sensitization, and grasp the information of individual differences in the appearance of adverse reactions in further research.
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PMID:Nickel-based (Ni-Cr and Ni-Cr-Be) alloys used in dental restorations may be a potential cause for immune-mediated hypersensitivity. 1948 41

The histamine H1-receptor (H1R) is an important mediator of allergy and inflammation. H1R antagonists have particular clinical utility in allergic rhinitis and urticaria. Here we have developed six novel fluorescent probes for this receptor that are very effective for high resolution confocal imaging, alongside bioluminescence resonance energy transfer approaches to monitor H1R ligand binding kinetics in living cells. The latter technology exploits the opportunities provided by the recently described bright bioluminescent protein NanoLuc when it is fused to the N-terminus of a receptor. Two different pharmacophores (mepyramine or the fragment VUF13816) were used to generate fluorescent H1R antagonists conjugated via peptide linkers to the fluorophore BODIPY630/650. Kinetic properties of the probes showed wide variation, with the VUF13816 analogues having much longer H1R residence times relative to their mepyramine-based counterparts. The kinetics of these fluorescent ligands could also be monitored in membrane preparations providing new opportunities for future drug discovery applications.
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PMID:Development of novel fluorescent histamine H1-receptor antagonists to study ligand-binding kinetics in living cells. 2937 69