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Query: UMLS:C0040822 (tremor)
 18,428 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.
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PMID:A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles. 12 53

We characterize two assays of total amniotic fluid surfactant that are based on function: the surface-tension lowering ability of extracts of amniotic fluid lipid (I) and the foam stability index test (II). I is determined on chloroform extracts of amniotic fluid. II is defined as the highest ethanol volume fraction of an amniotic fluid-ethanol mixture that will permit a stable foam to form after 30 s of vigorous shaking. The relationship of I to disaturated phosphatidylcholine concentrations (after osmium tetroxide treatment of the amniotic fluid lipid extract) is in the expected theoretical form of a hyperbolic function. The relation between values for II and disaturated phosphatidylcholine concentrations showed a consistent bias, suggesting that components other than disaturated phosphatidylcholine contribute to stable foam formation. Phosphatidylclycerol concentrations did not appear to account for this bias. The relation between I to II values suggest that both assays measure total surfactant. I, II, and concentration of disaturated phosphatidyl choline are all excellent indicators of fetal pulmonary maturity. From a practical standpoint, the foam stability index test is the most efficient approach to routine assessment of fetal pulmonary status.
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PMID:Evaluation of two assays of functional surfactant in amniotic fluid: surface-tension lowering ability and the foam stability index test. 58 78

The rate of denaturation of hemaglobin and other proteins by mechanical shaking is strongly affected by organic solvents. A group pf solvents,including alcohols and ketones, was found to stabilize proteins at low concentrations, although these same organic solvents denatured proteins at high concentrations. The stabilizing effect of alcohols increased with increasing chain lenghts. The second group of solvents, including toluene and chloroform, showed only a destabilizing effect, while the third group of solvents such as formamide and pentane had no effect over a wide range of concentrations. Organic solvents may be used to protect or denature a specific protein in solutions containing various proteins.
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PMID:Stabilizing effect of various organic solvents on protein. 68 59

Some 10% and lower guarantee carbaryl dusts cannot be quantitatively extracted by shaking with chloroform as specified in the official first action infrared method for carbaryl formulations, 6.206-6.208. A repeatability extraction study has been made, using a different extraction technique with both chloroform and 10% methanol in chloroform. The results indicate that the new extraction technique and the mixed solvent are suitable for all solid carbaryl formulations. It is recommended that a collaborative study be conducted on the new solvent system and extraction technique.
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PMID:Carbaryl insecticide: extraction from dust and powder formulations. 80 73

A simple and rapid high-performance liquid chromatographic procedure is described for the determination of strychnine. Grain baits containing strychnine alkaloid are ground, mixed, and extracted by shaking with chloroform. Without further cleanup, extract filtrates are injected directly into a liquid chromatograph. Chromatography is complete within 7 min and peak heights are used for quantitation. Separations were made on a 30 cm times 4 mm id stainless steel column packed with mu Porasil (8-12 mum silica). The eluting solvent was methanol-chloroform (10+90) at a flow rate of 2.0 ml/min. Recovery of spiked samples ranged from 91.5 to 95.2%. Confirmation of strychnine from a commercial sample was made by high resolution mass spectrometry with mass agreement to 1.2 ppm.
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PMID:High-performance liquid chromatographic determination of strychnine in grain baits.l. 115 38

A simple and rapid gas-liquid chromatographic procedure, using a 6' times 1/4'' glass column packed with 5% SE-30 on Chromosorb W (DMCS) and a flame ionization detector, is described. Grain baits containing strychnine alkaloid are ground, mixed, and extracted by shaking with chloroform containing an internal standard, 1,3,5-triphenylbenzene. Without further cleanup, extract filtrates are injected directly into a gas chromatograph. Peak height ratios are used for quantitation of strychnine. The analysis of commercial samples shows that the method compares well with a commonly employed ultraviolet spectrophotometric method; good precision, with recoveries ranging from 89.9 to 91.7%, is obtained in the analysis of prepared samples. The method is sensitive to 2 mug strychnine.
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PMID:Gas-liquid chromatographic determination of strychnine in grain baits. 115 39

I present a new, simple colorimetric method for detecting and estimating barbiturates in urine. After the barbiturates are extracted with ether, an aliquot of the washed ether phase is added to the color reagent (a bivalent mercury/dithizone chelate in chloroform). On addition of diluted pyridine and shaking, a pinkish-violet color appears if a barbiturate is present. The overall sensitivity of the above method was evaluated by probit analysis in the case of sodium phenobarbital. The concentration of sodium phenobarbital in urine detectable at least 99% of the time was 6.72 mg/liter, with 95% confidence limits of 5.37 to 10.36 mg/liter. Sodium phenobarbital, 10 mg/liter, can be detected in the presence of phenytoin (50 mg/liter), glutethimide (100 mg/liter), or bemegride (100 mg/liter). The whole procedure requires less than 10 min. An excretion study illustrates application of the procedure.
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PMID:Simple, highly selective screening method for barbiturates in urine. 116 89

By shaking a dilute suspension of egg yolk with chloroform followed by low speed centrifugation (1500 g for 30 min) the water soluble proteins which include chicken IgG (IgY) separate from the emulsion of chloroform and lipophilic substances. The IgY may then be separated from the associated water soluble proteins by precipitation with 12% polyethylene glycol Mr 6000. The method called the chloroform - polyethylene glycol procedure was compared with the polyethylene glycol procedure which is currently being used. It was found that the chloroform - polyethylene glycol method yielded 2.57 times more IgY than the conventional polyethylene glycol method. The ratio of titres of IgY anti Jasus lalandii haemocyanin antibody purified by the two procedures was very nearly 2.57 indicating that the chloroform had no adverse effect on the antibody activity.
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PMID:Isolation of IgY from the yolks of eggs by a chloroform polyethylene glycol procedure. 236 27

A rapid procedure has been developed for the analysis of phenylalanine in brain tissue. Perchloric acid extracts of brain tissue were made basic, and benzoyl chloride was added to derivatize the amine function. The aqueous layer was retained and made slightly acidic. To derivatize the carboxylic acid group, a solution of pentafluorobenzyl alcohol was added in the presence of the coupling agent dicyclohexylcarbodiimide and chloroform. After shaking for 15 min, the organic phase was retained and taken to dryness. The residue was taken up in toluene, washed, and an aliquot used for analysis on a gas chromatograph equipped with an automatic injector, a capillary column and an electron-capture detector. The procedure has been utilized for analysis of phenylalanine in brains of rats treated with vehicle or phenylalanine.
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PMID:A rapid procedure for the analysis of phenylalanine in brain tissue utilizing electron-capture gas chromatography. 236 72

A method is described for the removal of contaminating hemoglobin from the peroxidase enzyme in traumatic skin lesions. The procedure is based on hemoglobin precipitation in a combination of ammonium sulfate half-saturation, and chloroform shaking of the cetyltrimethylammonium-bromide extract. The procedure as such somewhat increases the activity of the peroxidase extract if the extract contains no hemoglobin. On the other hand, the peroxidase activity of the extract decreases as the amount of precipitating hemoglobin increases. On average, about 90% of the peroxidase activity persists after hemoglobin precipitation if the hemoglobin concentration in the extract does not exceed 25 mg/100 ml. In experimental incision wounds, the peroxidase activities obtained with this procedure were the same as when enzyme determinations were done without the removal of hemoglobin or slightly higher. In addition, the amount of peroxidase activity in the wounds was estimated, based on the granulocytes of the contaminating blood.
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PMID:Removal of contaminating hemoglobin from peroxidase in traumatic skin lesions. 255 63


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