Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0040822 (tremor)
 18,428 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A second case of Hb York (beta 146 His leads to Pro), was discovered in a patient with polycythemia. The oxygen equilibrium curves (OEC) of red cell suspensions in a buffer (pH 7.4) at 37 degrees C revealed a biphasic curve with a P50 of only 12.5 mm Hg (normal value: 26.5 +/- 1.0 mm Hg). The purified Hb York had an extremely high affinity for oxygen with diminished cooperativity and decreased Bohr effect. The oxygen affinity was significantly reduced by inositol hexaphosphate. Molecular stability studies by mechanical shaking of various liganded forms of Hb York revealed stabilities between those of Hb A and Hb S. Isolated beta Y-subunits were more unstable than beta A-subunits at every pH examined. Hb York was 1.4 times more unstable than Hb A in 18.9% isopropanol.
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PMID:Characterization and properties of Hb York (beta 146 His leads to Pro). 687 72

Fortified milks were saponified overnight at room temperature with 1% ethanolic pyrogallol and KOH. The digest was extracted with hexane after adding water and ethanol, and the extract was washed consecutively with 5% KOH, water, and 55% aqueous ethanol to remove polar lipids. After evaporation, the residue was first chromatographed on a column of 5 micrometer silica. A fraction containing vitamin D was collected, evaporated, and rechromatographed on a reverse phase column for the separation and quantitation of vitamins D2 and D3. Recovery was 96-99% and the coefficient of variation was 3% (8 replicates). Infant formula was diluted and then saponified and extracted as in the analysis of milk. Margarine was saponified by shaking overnight with 1% ethanolic pyrogallol and 80% KOH. Water and ethanol were added to the digest before extraction. Extracts from formula and margarine were chromatographed as milk except, before HPLC, the extract was dissolved in isopropanol-hexane (1 + 99) and passed through 5 cm alumina in a Pasteur pipet, and the concentration of isopropanol in the first high performance liquid chromatographic (HPLC) solvent system was halved to improve the separation of vitamin D from other absorbing lipids. Usually several peaks were obtained during the final HPLC analysis, and the identification of vitamins D2 and D3 was less certain than in the analysis of milk. The coefficients of variation for formula and margarine were 6% (5 replicates) and 9% (6 replicates), respectively.
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PMID:High performance liquid chromatographic determination of vitamin D in fortified milks, margarine, and infant formulas. 709 44

Exposure to HBO causes hypothermia, bradycardia, head weaving, resting tremor, piloerection, and straub tail in rats. These physiological and behavioral responses can also be evoked by selective activation of serotonin1A (5-HT1A) receptors. The purpose of the current study was to determine if hypothermia caused by HBO is due to increased activation of 5-HT1A receptors. The levels of brain biogenic amines were measured in brain regions of Sprague-Dawley (SD) rats exposed to HBO. Exposure to HBO caused an increase in the levels of 5-hydroxyindoleacetic acid (5-HIAA) in the striatum (92%, p < 0.05) and occipital-temporal cortex (116%, p < 0.05), but not in other brain regions. Exposure to HBO did not change the levels of tryptophan, serotonin (5-HT), other biogenic amines, or their metabolites. It is hypothesized that the Fawn Hood (FH) rat, which is reported to be resistant to hypothermia induced by 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), has an abnormality of 5-HT1A receptor activity. Although the FH rat was more resistant to hypothermia induced by HBO than the SD rat, we were not able to confirm that this rat was resistant to hypothermia induced by 8-OH-DPAT. The 5-HT receptor antagonists, 1-(1H-Indol-4-yloxy)-3-[(1-methylethyl)amino]-2-propanol (Pindolol), 1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl] piperazine hydrobromide (NAN-190), and methysergide, did not block hypothermia induced by HBO in SD rats. A series of control experiments were used to confirm that the antagonists blocked hypothermia induced by serotonin agonists.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Hypothermia induced by hyperbaric oxygen is not blocked by serotonin antagonists. 844 68

A rapid normal-phase high-performance liquid chromatographic method for the quantitative determination of indole and skatole in pig back fat samples has been developed. The compounds were extracted by shaking the samples at ambient temperature in hexane-2-propanol (92:8, v/v). The sample preparation procedure was simple because it was not necessary to remove the fat from the samples. The compounds were separated on a 250 x 4.6 mm I.D., 5 micron Hypersil aminopropylsilica column. Fluorescence (excitation at 280 nm and emission at 360 nm) was used for selective detection. Recoveries for skatole and indole, relative to the internal standard, were 10.3 +/- 0.9% and 99.6 +/- 4.4%, respectively. Linearity determined in fat samples was in the range of 0.05-1 microgram/g and the correlations observed were R2 = 0.9914 for the indole and R2 = 0.9916 for skatole.
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PMID:Rapid determination of skatole and indole in pig back fat by normal-phase liquid chromatography. 967 18

Sampling and analytical methods were developed for commonly used chloroacetanilide, chlorotriazine, and 2,4-D herbicides in hand washes, on dermal patches, and in air. Eight herbicides selected for study were alachlor, atrazine, cyanazine, 2,4-dichlorophenoxyacetic acid (2,4-D), metolachlor, simazine, and two esters of 2,4-D, the 2-butoxyethyl ester (2,4-D, BE) and the 2-ethylhexyl ester (2,4-D, EH). The hand-wash method consisted of shaking the worker's hand in 150 mL of isopropanol in a polyethylene bag for 30 seconds. The dermal-patch method entailed attaching a 10-cm x 10-cm x 0.6-cm polyurethane foam (PUF) patch to the worker for exposure; recovery of the herbicides was achieved by extraction with 40 mL of isopropanol. The air method involved sampling with an OVS-2 tube (which contained an 11-mm quartz fiber filter and two beds of XAD-2 resin) and recovery with 2 mL of 10:90 methanol:methyl t-butyl ether. Analysis of each of the three sample types was performed by gas chromatography with an electron-capture detector. Diazomethane in solution was employed to convert 2,4-D as the free acid to the methyl ester in each of the three methods for ease of gas chromatography. Silicic acid was added to sample solutions to quench excess diazomethane. Limits of detection for all eight herbicides were matrix-dependent and, generally, less than 1 microgram per sample for each matrix. Sampling and analytical methods met NIOSH evaluation criteria for all herbicides in hand-wash samples, for seven herbicides in air samples (all herbicides except cyanazine), and for six herbicides in dermal-patch samples (all herbicides except cyanazine and 2,4-D). Speciation of 2,4-D esters and simultaneous determination of 2,4-D acid were possible without losses of the esters or of other herbicides (acetanilides and triazines) being determined.
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PMID:Development of sampling and analytical methods for concerted determination of commonly used chloroacetanilide, chlorotriazine, and 2,4-D herbicides in hand-wash, dermal-patch, and air samples. 1141 20

Parkinson's disease (PD) is clinically characterized by a resting tremor, bradykinesia, cogwheel phenomenon, rigidity, disorder of postural reflexes and especially changes in voice and speech. We studied 30 PD patients who were treated with dopamine and 20 normal subjects as the control group. The parameters of vocal fold edges, glottal closure, vertical levels of cords, amplitude of vibration, mucosal wave, vibratory behavior, phase symmetry, ventricular folds and movements, periodicity, arytenoids and thick mucous were evaluated by videolaryngostroboscopy. The Unified Parkinson's Disease Rating Scale was applied to the patient group. The voices of the patients were evaluated by the Dr.Speech-4 and Spectra-PRO computer programs. Maximum phonation time, fundamental frequency, amplitude and the harmonic-to-noise ratio were recorded and compared with those of the control group. The abnormal videolaryngostroboscopic findings were more frequent in the PD group (70% versus 45%; P<0.05). Voice analysis showed significant differences in the parameters such as maximum phonation time, maximum fundamental frequency, the frequency range and the harmonic-to-noise ratio. We thought that these methods and parameters yielded sufficient information for diagnosis and follow-up of vocal function in patients with PD.
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PMID:Voice analysis and videolaryngostroboscopy in patients with Parkinson's disease. 1211 74

The aim of this study was to investigate whether two different brands of unsupported and unlined nitrile gloves protected against aqueous emulsions of a Folpet wettable powder (50% Folpet) using an ASTM type-I-PTC 600 permeation cell at 30.0 +/- 0.1 degrees C held in a shaking water bath. An analytical method to determine Folpet using the internal standard method was first developed based on gas chromatography-mass spectrometry (GC-MS), and gas chromatography-electron capture detection (GC-ECD). A novel pyrolysis GC-ECD technique that quantified the thermal degradation product phthalimide had pg sensitivity suitable to detect the trace amounts of Folpet that permeated. The on-column conversion was (68.0 +/- 9.5) percent at 170 degrees C over the folpet injected mass range of 3 to 148 pg. The challenge solution in the permeation cell was 1.4 mg/mL aqueous emulsion of Folpet wettable powder, and 2-propanol was the collection solvent. After evaporation of the collection solvent, the time weighted average rate of permeation of Folpet through SafeSkin nitrile (an exams type of glove) after 8 hours was (42.1 +/- 2.9) ng/cm(2)/min compared with (2.04 +/- 0.69) ng/cm(2)/min for the Sol-Vex nitrile (industrial chemical resistant), the latter being about 21 times more protective and also near the limits of detection. The respective values after 4 hours of exposure were (28.4 +/- 1.2) and (0.65 +/- 0.36) ng/cm(2)/min. Diagnostic reflectance infrared minima of both challenge and collection sides of the gloves showed small changes in wave number and intensity values after 8 hours of exposure, with Folpet being detected in dried spots on the challenge side. GC-ECD-based permeation and IR reflectance data indicated high chemical resistance of the Sol-Vex gloves to an aqueous emulsion of Folpet.
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PMID:Folpet permeation through nitrile gloves. 1290 34

(R)-chlorprenaline, a selective activator of beta2 receptor and an effective drug for bronchitis and asthma, is industrially prepared from (R)-2'-chloro-1-phenyl-ethanol. In this communication, we describe (1) the identification of Saccharomyces cerevisiae B5 as an effective host for stereoselective reduction of 2'-chloroacetophenone to (R)-2'-chloro-1-phenyl-ethanol; (2) the presence of ethanol enhances the conversion; and (3) the biochemical factors that effect the yield of the product. Among the four yeast strains capable of reduction 2'-chloroacetophenone to (R)-2'-chloro-1-phenyl-ethanol we screened, Saccharomyces cerevisiae B5 showed the highest activity and stereoselectivity, and was used for the subsequent study. The effect of the presence of methanol, ethanol, 2-propanol, 1-butanol, glucose, glycerol and lactic acid was first investigated, as it was previously reported that they increased the yield and stereoselectivity of the reaction. The addition of the co-substrate methanol, ethanol, 2-propanol, 1-butanol, glucose and glycerol favored the formation of the 2'-chloroacetophenone to (R)-2'-chloro-1-phenyl-ethanol. Lactic acid inhibited the enzyme activity. Ethanol is the best co-substrate among the seven co-substrates and under the optimum concentration of 5% , the yield of (R)-2'-chloro-1-phenyl-ethanol was increased from 17% to 74%. The oxidation of ethanol regenerates NADH required for the reduction. The effects of the reaction time, pH, cell concentration, substrate concentration and temperature on the reduction were investigated next. The enantiometric excess of (R)-2'-chloro-1-phenyl-ethanol reached 100% under the optimal condition: pH8.0, 25 degrees C and 5% ethanol. The product yield went up with the increasing Saccharomyces cerevisiae B5 concentration and reached 100% when the cell dry weight was 10.75 mg/mL and 2'-chloroacetophenone was 6.47 mmol/L. The yield of (R)-2'-chloro-1-phenyl-ethanol decreased sharply with the increase of substrate concentration, as the high concentration of substrates is toxic to the cell and inhibits the activity of reductases. The aerobic cultivation of the yeast and shaking during the reaction increased the yield of (R)-2'-chloro-1-phenyl-ethanol. The yeast can be reused up to 15 times. This research paves the way for economical preparation of chiral 2'-chloroacetophenone to R-2'-chloro-1-phenylethanol.
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PMID:[Saccharomyces cerevisiae B5 efficiently and stereoselectively reduces 2'-chloroacetophenone to R-2'-chloro-1-phenylethanol in the presence of 5% ethanol]. 1596 23

A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by vigorously shaking the washed soil in a 4 M guanidine thiocyanate solution containing 25 mM sodium citrate, 0.5% sarcosyl, and 0.1 M 2-mercaptoethanol. The pH of the homogenized mixture was adjusted with 2 M sodium acetate (pH 4.0); the mRNA was then extracted with phenol and chloroform. Total RNA was precipitated with isopropanol. This method extracts up to 17 mug of total RNA per g (wet weight) of soil containing 8.0 x 10 cells of Pseudomonas aeruginosa PU21, and mRNA has been detected in 160-ng total RNA fractions. This method has been used for the detection of mRNA transcribed from specific biodegradative genes, including the nah and mer operons, in contaminated soils. This extraction method can be completed within a few hours and has tremendous potential for ecological studies of in situ gene expression among soil microbiotas.
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PMID:Rapid method for direct extraction of mRNA from seeded soils. 1634 41

The high-performance liquid chromatography with electrochemical detection (HPLC-EC) of six anthracycline antibiotics is described. All the compounds of interest may be analysed using a reversed-phase column (ODS Hypersil or Zorbax ODS) and a mobile phase consisting of acetonitrile-isopropanol-0.1 M phosphate buffer (pH 4.5). The use of short columns (7.5 cm) allowed short analysis times and improved sensitivity, such that detection limits of between 1 and 2 ng ml(-1) could be achieved. Sample preparation involved an alkaline extraction into chloroform, followed by a back extraction into acid solution. Final clean-up of the samples is achieved by shaking the final acidic extract with an organic solvent. The precision of the assay for the compounds studied ranges between 1.22 and 6.46% and the accuracy ranges between 94.5 and 106.0%. The analytical methodology developed is applied to the clinical pharmacokinetics of 4'-deoxydoxorubicin. The correlation between plasma levels found by HPLC-EC and those found by HPLC with fluorescence detection is excellent (r(2) = 0.990).
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PMID:High-performance liquid chromatography of anthracycline antibiotics with electrochemical detection. Application to the clinical pharmacokinetics of 4'-deoxydoxorubicin. 1686 44


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