Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
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Target Concepts:
Gene/Protein
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Query: UMLS:C0040822 (
tremor
)
18,428
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An RFB (radial-flow bioreactor) with a reactor volume of 5 ml was applied to produce human beta3GnT (beta1,3-
N-acetylglucosaminyltransferase
) using two stably transformed insect cell lines. When air was supplied to the RFB, cell growth stopped at 4 days of culture and beta3GnT was not detected. However, with a supply of pure oxygen, the cell concentration, assumed from glucose consumption, increased by 1.3x10(7) cells/ml. Insect cells attached to poly(vinyl alcohol) matrixes packed in the RFB and grew confluently; 5.6 m-units/ml beta3GnT was produced under the conditions of pure oxygen supply and addition of glucose and glutamine. This RFB was first applied in beta3GnT production using stably transformed insect cells. The amount of beta3GnT production in only a 5 ml-scale RFB was comparable with that of a 100 ml
shaking
flask culture.
...
PMID:Application of a radial-flow bioreactor in the production of beta1,3-N-acetylglucosaminyltransferase-2 fused with GFPuv using stably transformed insect cell lines. 1563 81
