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Query: UMLS:C0040822 (
tremor
)
18,428
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Dispersed rat anterior pituitary cells were allowed to reassociate into spherical aggregates by gyrotory
shaking
in serum-free chemically defined culture medium. When aggregates were superfused after being cultured for 5 days in this medium, stimulation of
PRL
release by TRH, VIP, angiotensin II and the beta-adrenergic agonist isoproterenol was comparable to that of aggregates cultured in serum-supplemented culture medium. Addition to the serum-free medium of 80 nM dexamethasone (Dex) resulted in a significant enhancement of the stimulation of
PRL
release by TRH, VIP and angiotensin II but not of the stimulation of
PRL
release by isoproterenol. Dex also failed to influence the inhibition of
PRL
release by 10 min exposure to 10 nM dopamine (DA). However, Dex significantly enhanced the post-DA rebound secretion of
PRL
. After 3 weeks in culture Dex provoked a similar potentiation of the response to angiotensin as at 5 days in culture but it abolished almost completely the stimulatory effect of isoproterenol. It is concluded that pituitary cell aggregates cultured in defined serum-free medium are a reliable system to study the multifactorial control of
PRL
release. The data show that peptidergic, dopaminergic and beta-adrenergic control at the pituitary level is differentially modulated by corticosteroids.
...
PMID:Influence of corticosteroids on prolactin release from anterior pituitary cell aggregates cultured in serum-free medium. Differential effects on dopamine-induced inhibition, post-dopamine rebound and stimulation by TRH, vasoactive intestinal peptide (VIP), angiotensin II and isoproterenol. 642 98
Suspensions of isolated rat pituitary cells and gonadotroph- or lactotroph/somatotroph-enriched subpopulations obtained by unit gravity sedimentation were allowed to aggregate by constant gyrotory
shaking
, yielding aggregates of 100-150 micrometers. Within a few days, the aggregated pituitary cells became organized in a tissue-like configuration. There was no proliferation of mesenchymal cells. Glandular cells had a round to oval shape and formed specialized cell junctions. Areas of close apposition alternated with more dilated intercellular spaces. The different pituitary cell types retained their characteristic ultrastructural features and secretory granules, typical of the various cell types. Functional characteristics could be accurately studied in a superfusion system. LH, FSH, and
PRL
secretion showed very rapid on-off responses to nanomolar concentrations of the specific regulatory stimuli LHRH, TRH, vasoactive intestinal peptide, and dopamine. Aggregates remained highly responsive to LHRH even after 3 weeks in culture. The results indicate that isolated pituitary cells allowed to reaggregate in suspension culture form viable and functional multicellular structures which have maintained in vivo characteristics.
...
PMID:Ultrastructural and functional characteristics of rat pituitary cell aggregates. 679 74
