Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0039483 (giant cell arteritis)
 3,204 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

PUVA effects on frozen sections of human epidermis were investigated (20 micrograms 8-methoxypsoralen/ml; UVA wavelength 365 +/- 5 nm; radiation dose between 2 X 10(2) and 2 X 10(2) J/m2). The quality of the glycocalyx was characterized by lectins labeled with FITC (HPA, PHA, LCA, PNA, SBA, RCA, GCA I, UEA I). Serum antibodies of bullous pemphigoid (BP) served as membrane markers for the basement membrane. Fixed BP antibodies were detected by FITC-antihuman IgG. The fluorescence intensity was markedly decreased at a dose of 2 X 10(5) J/m2; 2 X 10(4) J/m2 were less effective. At 2 X 10(3) J/m2, there was nearly no alteration of the fluorescence intensity observed. Changes were seen in the case of BP antibodies and lectins, except UEA I. The relatively high intensity (radiation dose) and the effects immediately observable suggest direct membrane alterations by PUVA in vitro.
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PMID:[PUVA irradiation decreases the binding of membrane and basement membrane markers to human epidermis in vitro]. 331 11

The binding of five different lectins by normal as well as psoriatic lesional epidermis was investigated with regard to its dependence on the pH value. As we studied the reactions within the pH range of 3.0 to 9.66, we obtained the following results: lectin binding almost independent from pH (FITC-Con A, -PHA, HPA), fluorescence intensity dependent on pH (TRITC-Con A, FITC-LCA, -GCA), binding pattern dependent on pH (FITC-LCA, GCA). In contrast to normal epidermis, there was no pH dependence of the FITC-GCA binding pattern in psoriatic lesional epidermis.
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PMID:[Characterization of lectin binding by psoriatic epidermis--dependence on pH]. 357 86