Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0038454 (stroke)
 147,016 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A chemical quenched-flow apparatus is described which measures, in a unique stroke, enough data points (8-11) for establishing the kinetics curve of a reaction. Only very small volumes of reaction solutions (2 X 500 microliters) are required. The time intervals between which the kinetic data may be measured range from 5 to 37 ms and from 120 to 450 ms with the corresponding mixing times of 0.6 and 5 ms, respectively. This apparatus was used to investigate the pre-steady-state domain of the aminoacylation reaction of tRNAVal by valyl-tRNA synthetase from yeast.
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PMID:A quenched-flow apparatus which allows the measurement of the kinetics of a reaction in one stroke. 623 52