Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0038362 (stomatitis)
 8,852 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The importance of electrostatic interactions in the early phases of vesicular stomatitis virus (VSV) infection has been investigated in susceptible cells of different origin, human (HeLa) and avian (CER), by using some polyanions (heparin, polygalacturonic acid and mucin) and polycations (polymyxin B sulphate, poly-L-lysine, protamine, histone and polybrene). In HeLa cells, the attachment of VSV was enhanced by polymers having a positive charge and inhibited by those having a negative charge. In CER cells, all the polyanions tested reduced virus infection. Among the polycations, histone, polymyxin B sulphate and poly-L-lysine enhanced virus plaque formation while protamine and polybrene reduced virus attachment. The effect of polyions on VSV particles and on cell membrane receptors has also been investigated. The analysis of the results obtained suggest that, although electrostatic interactions play an essential role in the binding of VSV to the cell membrane, more specific structural features appear to be required for viral attachment to occur.
 ...
PMID:Electrostatic interactions in the early events of VSV infection. 164 50

To identify membrane components of CER cells interacting with vesicular stomatitis virus (VSV) during fusion at acidic pH (fusion from without, FFWO) two different approaches have been used, i.e. (i) treating the whole cells with enzymes and (ii) testing the ability of isolated membrane molecules to interfere with FFWO. Phospholipase A2 and C digestion of cells greatly reduced syncytia formation, pointing towards the involvement of lipid structures as target sites for VSV. Cell susceptibility to FFWO was also reduced after neuraminidase, beta-galactosidase or periodate treatment, suggesting that carbohydrate residues may participate in a complex receptor structure required for virus fusion. When membrane molecules were examined separately for their ability to inhibit viral FFWO, phosphatidylserine, phosphatidylinositol, sphingomyelin, cholesterol and GM3 ganglioside were found to be active, confirming the role of membrane lipid moiety in the cell surface structures involved in the early phases of VSV infection.
 ...
PMID:Role of membrane phospholipids and glycolipids in cell-to-cell fusion by VSV. 166 Jul 97

The effect of some cellular function inhibitors on adsorption and successive events of penetration of vesicular stomatitis virus to phylogenetically unrelated permissive cells was investigated. Treatment of HeLa, CER, EPC and Aedes albopictus cells with colcemide and cytochalasin D which affect cytoskeleton organization indicated that microfilaments but not microtubules were involved in the early events of VSV infection. Inhibitors of oxidative phosphorylation such as dinitrophenol and sodium azide, and the glycolysis inhibitor 2-deoxy-D-glucose, did not allow multiplication of prebound virions, demonstrating that VSV replication is largely energy dependent in either host cells examined.
 ...
PMID:Effect of cellular inhibitors on the infection of various susceptible cells with vesicular stomatitis virus. 290 20

Research was carried out on the adherence of a mannose-resistant uropathogenic E. coli strain to CER cells infected with vesicular stomatitis virus (vsv). A decrease in the bacterial adhesion was noticed during the early phases of viral infection, probably due to a close relationship between cell receptors for VSV and E. coli, both containing glycolipids and phospholipids. In a later phase of viral infection, on the contrary, corresponding to the appearance on the cell surface of newly synthesized viral antigens, bacterial adherence was enhanced. This last observation suggested a possible predisposition of the host to bacterial colonization during renal viral infection.
 ...
PMID:E. coli adherence to CER cells infected by vesicular stomatitis virus. 298 86

In the present report an attempt was made to elucidate the role of gangliosides in early interactions between vesicular stomatitis virus (VSV) and CER cells. Research was carried out to test the ability of gangliosides from mammal brains and from CER cells to inhibit viral attachment to susceptible cells. The incubation of VSV in the presence of gangliosides decreased the subsequent infection of CER cells by the virus. When similar experiments were performed with gangliosides inserted in liposomes the inhibition of infection was enhanced. Since carbohydrate moieties could participate to rhabdovirus binding as a part of a glycolipid receptor, CER cells were subjected to the action of glycosidases and these produced a fall in the viral attachment. Deglycosilated CER cells reacquired their susceptibility to virus infection after coating with gangliosides immediately after enzyme treatment. Results obtained show the participation of gangliosides in the receptorial structure for vesiculovirus of susceptible CER cells.
 ...
PMID:Gangliosides in early interactions between vesicular stomatitis virus and CER cells. 299 65

Enveloped viruses enter host cells by fusion or viropexis. The latter mechanism is the prevalent entry pathway of rhabdoviruses into susceptible cells. Amantadine, a lysosomotropic agent, inhibits the multiplication of various groups of viruses. The effect of this drug was investigated on vesicular stomatitis virus and rabies fixed virus strain replication in fibroblasts. Amantadine was added to cells before, during and after infection to detect the phase of viral replication affected by the drug. Cells were inoculated with viruses at 4 degrees C and the incubation temperature was progressively raised to 37 degrees C in order to observe the effect of amantadine on attachment and early stages of viral replication. Experimental results indicated that the compound inhibited rhabdovirus infection in CER cells. Viral attachment and penetration did not appear to be affected by the drug, while later steps were inhibited, probably at the level of uncoating when the virus is released from the lysosomes into the intracytoplasmic compartment.
 ...
PMID:Effect of amantadine on rhabdovirus infection. 301 59

Early interactions between vesicular stomatitis virus (VSV) and susceptible cells were examined in cell lines of mammalian (HeLa), bird (CER), piscine (EPC) and arthropod (Aedes albopictus) origin showing different permissiveness to VSV growth. The chemical nature of receptors was investigated either by modification of cell surfaces with different enzymes or by competition for VSV binding between extracted membrane components and whole cells. Results obtained indicate that in all cell models, membrane lipid components show receptor activity whereas glycid groups participate to the in virus binding to a different extent.
 ...
PMID:Study of receptors for vesicular stomatitis virus in vertebrate and invertebrate cells. 301 49

A biochemical and morphological investigation of the mechanism of entry of vesicular stomatitis virus (VSV) into host cells of mammalian (HeLa), avian (CER), piscine (EPC) and arthropod (Aedes albopictus) origin, is described. VSV was capable of infecting all cell lines tested by a endosome- and/or a lysosome-dependent step since ammonium chloride and amantadine blocked the early stages of infection. Complement-dependent immune lysis of infected host cells provided evidence that in none of the four different cell types examined did insertion of VSV antigens occur from the outside to any great extent on the cell surface. When the entry process was studied by electron microscopy, virus particles were seen to be bound to the cell surface at 0 degrees C. After warming at 37 degrees C for homeothermic cells or at 26 degrees C for poikilothermic cells, virus was detected within coated pits and coated vesicles and, later, in lysosomes. VSV entry was seen to take place by endocytosis in all four cell lines, which were derived from phylogenetically unrelated species.
 ...
PMID:Entry pathway of vesicular stomatitis virus into different host cells. 302 82

The effect of phospholipases on rhabdovirus attachment to CER cells and the competitive binding of phospholipids and viruses to cells were studied. Results obtained indicate that, although to a different extent, some phospholipids represent a binding site for both Vesicular Stomatitis Virus (VSV) and rabies virus.
 ...
PMID:Role of phospholipids in rhabdovirus attachment to CER cells. Brief report. 608 6

CER cells infected with vesicular stomatitis virus showed a morphology similar to that observed after cytochalasin B treatment. Temperature-sensitive mutants affected in envelope protein maturation did not induce those morphological changes at a nonpermissive temperature. In addition, the cytoskeleton was not implicated in vesicular stomatitis virus reproduction.
 ...
PMID:Is cytoskeleton involved in vesicular stomatitis virus reproduction? 624 12


1