Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0038362 (stomatitis)
 8,852 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The relationship between the development of virus plaque forming cells (V-PFC) and the blastogenic response of T cells activated with concanavalin A (Con A) was investigated in connection with the cell cycle using a modified virus plaque assay (VPA) which facilitated the quantitative detection of T lymphoblasts with low susceptibility to infection by vesicular stomatitis virus (vsv). The generation of V-PFC markedly decreased when DNA synthesis inhibitors such as thymidine, hydroxyurea and cytosine arabinoside (Ara C) were added to spleen cell cultures stimulated with Con A, while the addition of a mitotic inhibitor, Colcemid, to the cultures caused only partial reduction of V-PFC development. These results indicate that the S to G2 phases of the first cell cycle are essential for vsv-replication or V-PFC development in Con A activated T cells. However, vsv-replication in the activated T cells does not depend on the initial G1 phase during the blastogenic response since the morphological blast formation of activated T cells is not suppressed by treatment with DNA synthesis inhibitors.
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PMID:Cell cycle requisite for vesicular stomatitis virus replication in T lymphocytes activated with concanavalin A. 632 97

The phosphoprotein (P) of vesicular stomatitis virus (VSV) is a subunit of the viral RNA polymerase. In previous studies, we demonstrated that insertion of 19 amino acids in the hinge region of the protein had no significant effect on P protein function. In the present study, we inserted full-length enhanced green fluorescent protein (eGFP) in frame into the hinge region of P and show that the fusion protein (PeGFP) is functional in viral genome transcription and replication, albeit with reduced activity. A recombinant vesicular stomatitis virus encoding PeGFP in place of the P protein (VSV-PeGFP), which possessed reduced growth kinetics compared to the wild-type VSV, was recovered. Using the recombinant VSV-PeGFP, we show that the viral replication proteins and the de novo-synthesized RNA colocalize to sites throughout the cytoplasm, indicating that replication and transcription are not confined to any particular region of the cytoplasm. Real-time imaging of the cells infected with the eGFP-tagged virus revealed that, following synthesis, the nucleocapsids are transported toward the cell periphery via a microtubule (MT)-mediated process, and the nucleocapsids were seen to be closely associated with mitochondria. Treatment of cells with nocodazole or Colcemid, drugs known to inhibit MT polymerization, resulted in accumulation of the nucleocapsids around the nucleus and also led to inhibition of infectious-virus production. These findings are compatible with a model in which the progeny viral nucleocapsids are transported toward the cell periphery by MT and the transport may be facilitated by mitochondria.
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PMID:Visualization of intracellular transport of vesicular stomatitis virus nucleocapsids in living cells. 1677 25