Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0038362 (
stomatitis
)
8,852
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Inflammatory lesions, often seen in diseases such as rheumatoid arthritis, atherosclerosis and cancer, feature an acidic (i.e., low pH) microenvironment rampant with cytokines, such as
CSF1
. For potential therapeutic intervention targeted at these
CSF1
sources, we have assembled a system of four proteins inside a cell (i.e., HEK293) that initially had no natural
CSF1
-seeking ability. This system included a newly engineered
CSF1
chimera receptor (named CSF1Rchi), the previously engineered Ca
2+
activated RhoA (i.e., CaRQ), vesicular
stomatitis
virus glycoprotein G (VSVG) and thymidine kinase (TK). The binding of
CSF1
to the CSF1Rchi generated a Ca
2+
signal that activated CaRQ-mediated cellular blebbing, allowing autonomous cell migration toward the
CSF1
source. Next, the VSVG protein allowed these engineered cells to fuse with the
CSF1
source cells, upon low pH induction. Finally, these cells underwent death postganciclovir treatment, via the TK suicide mechanism. Hence, this protein system could potentially serve as the basis of engineering a cell to target inflammatory lesions in diseases featuring a microenvironment with high levels of
CSF1
and low pH.
...
PMID:Autonomous Cell Migration to CSF1 Sources via a Synthetic Protein-Based System. 2847 71
