Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0038187 (
starvation
)
24,951
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
DIX domain containing 1
(
DIXDC1
), the human homolog of
coiled-coil-DIX1
(Ccd1), is a positive regulator of Wnt signaling pathway. Recently, it was found to act as a candidate oncogene in colon cancer, non-small-cell lung cancer, and gastric cancer. In this study, we aimed to investigate the clinical significance of
DIXDC1
expression in human glioma and its biological function in glioma cells. Western blot and immunohistochemistry analysis showed that
DIXDC1
was overexpressed in glioma tissues and glioma cell lines. The expression level of
DIXDC1
was evidently linked to glioma pathological grade and Ki-67 expression. Kaplan-Meier curve showed that high expression of
DIXDC1
may lead to poor outcome of glioma patients. Serum
starvation
and refeeding assay indicated that the expression of
DIXDC1
was associated with cell cycle. To determine whether
DIXDC1
could regulate the proliferation and migration of glioma cells, we transfected glioma cells with interfering RNA-targeting
DIXDC1
; investigated cell proliferation with Cell Counting Kit (CCK)-8, flow cytometry assays, and colony formation analyses; and investigated cell migration with wound healing assays and transwell assays. According to our data, knockdown of
DIXDC1
significantly inhibited proliferation and migration of glioma cells. These data implied that
DIXDC1
might participate in the development of glioma, suggesting that
DIXDC1
can become a potential therapeutic strategy for glioma.
...
PMID:Knockdown of DIXDC1 Inhibits the Proliferation and Migration of Human Glioma Cells. 2781 68
