Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0038187 (
starvation
)
24,951
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Chinemys reevesii
(Reeves' turtle) is a slow-growing reptile that is distributed widely across China. Prior to this study, the cDNA sequence of the growth hormone receptor (
GHR
) in the Reeve's turtle, or how periods of
starvation
might influence the gene expression of
GHR
and insulin-like growth factor I (
IGF-I
) in this species, were unknown. Here, we identified the full-length sequence of the cDNA encoding GHR in Reeves' turtle by using RT-PCR and RACE. The full-length
GHR
cDNA was identified to be 3936 base-pairs in length, with a 1848 base-pair open reading frame (ORF) that encodes a 615 amino acid protein. Analysis showed that
GHR
mRNA was detectable in a wide range of tissues; the highest and lowest levels of expression were detected in the liver and the gonad, respectively.
IGF-I
was also expressed in a range of tissues, but not in the gonad; the highest levels of
IGF-I
expression were detected in the liver. After 4 weeks of fasting, the expression levels of
GHR
and
IGF-I
in the liver had decreased significantly; however, these gradually returned to normal after refeeding. We report the first cloned cDNA sequence for the
GHR
gene in the Reeve's turtle. Our findings provide a foundation from which to investigate the specific function of the GHR in Reeve's turtle, and serve as a reference for studying the effects of different nutrient levels on
GHR
expression in this species.
...
PMID:Identification of the cDNA Encoding the Growth Hormone Receptor (
GHR
) and the Regulation of
GHR
and
IGF-I
Gene Expression by Nutritional Status in Reeves' Turtle (
Chinemys reevesii
). 3258 98
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