Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0038187 (
starvation
)
24,951
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Assimilation of short-chain fatty acids (SCFAs) plays an important role in the survival and lipid biosynthesis of
Mycobacteria
. However, regulation of this process has not been thoroughly described. In the present work, we demonstrate that GlnR as a well-known nitrogen-sensing regulator transcriptionally modulates the AMP-forming propionyl-CoA synthetase (MsPrpE), and acetyl-CoA synthetases (MsAcs) is associated with SCFAs assimilation in
Mycobacterium smegmatis
, a model
Mycobacterium
. GlnR can directly activate the expression of
MsprpE
and
Msacs
by binding to their promoter regions based upon sensed nitrogen
starvation
in the host. Moreover, GlnR can activate the expression of
lysine acetyltransferase
encoding
Mspat
, which significantly decreases the activity of MsPrpE and MsAcs through increased acylation. Next, growth curves and resazurin assay show that GlnR can further regulate the growth of
M. smegmatis
on different SCFAs to control the viability. These results demonstrate that GlnR-mediated regulation of SCFA assimilation in response to the change of nitrogen signal serves to control the survival of
M. smegmatis
. These findings provide insights into the survival and nutrient utilization mechanisms of
Mycobacteria
in their host, which may enable new strategies in drug discovery for the control of tuberculosis.
...
PMID:GlnR-Mediated Regulation of Short-Chain Fatty Acid Assimilation in
Mycobacterium smegmatis
. 2998 77
