Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
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Gene/Protein
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Target Concepts:
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Query: UMLS:C0038187 (
starvation
)
24,951
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A technique for detection of the activity of
hydroxylamine oxidoreductase
(
HAO
) involving denaturing SDS-polyacrylamide gels was developed. The activity of
HAO
of Nitrosomonas europaea was assayed using this technique, which revealed a single active band of 140 kDa. The
HAO
activity of other ammonia-oxidizers was also resistant to SDS, the molecular weights being identical to that of N. europaea. N. europaea cells starved of ammonia for up to 72 h retained a considerable amount of
HAO
, as detected on Western blot analysis, and a significant level of its activity, as found on assaying at the end of the
starvation
period. Only after 4 h incubation of starved N. europaea cells with 2.0 mM ammonia was some increase in the
HAO
level observed. The results indicate that
HAO
remains highly stable during ammonia
starvation
of N. europaea.
...
PMID:Effect of ammonia starvation on hydroxylamine oxidoreductase activity of Nitrosomonas europaea. 919 39
Axenic cultures of the ammonia-oxidizing bacterium Nitrosomonas europaea were starved of ammonia (energy source) for up to 342 d. During this time the bacteria retained the ability to respond instantly to ammonia (1 mM) or hydroxylamine (0.1 mM) amendment by oxidizing it to nitrite without initial protein synthesis. In vivo, the ability to oxidize amended ammonia stayed almost constant during the
starvation
period, but a drop in the hydroxylamine oxidation rate (to 33%) was observed after 4 wk of
starvation
when exogenous hydroxylamine was supplied as sole energy source. In contrast, it has been shown that the level and in vitro activity of
hydroxylamine oxidoreductase
were not significantly affected during the
starvation
period. Only minor changes were detected between the protein patterns on one-dimensional SDS-PAGE of growing and starved cells. Thus, it is concluded that the activities of the energy-generating enzymes in N. europaea were not affected during long-term ammonia
starvation
.
...
PMID:Effect of long-term ammonia starvation on the oxidation of ammonia and hydroxylamine by Nitrosomonas europaea. 975 28
