Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0038187 (starvation)
 24,951 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Vascular smooth muscle cells (V-SMC) alter their functions from contraction to production of extracellular matrices during the response to injury of the arterial wall. Accumulation of these matrices characterize the chronic structural change of arteriosclerosis. In this paper, we reported as follows. (1) V-SMC freshly isolated from human aortic media had strong reactivity with anti-smooth muscle specific alpha-actin (alpha-SM actin) antibody but not with anti-prolyl 4-hydroxylase (ProHy) antibody. (2) The exponentially growing cells in serum-containing culture media reduced alpha-SM actin and induced ProHy distinctly. (3) Growth-arrested cells with serum starvation rearranged numerous alpha-SM actin fibers in their cytoplasm. And the rearrangement of contractile filaments inhibited by the addition of single competence growth factor, such as platelet derived growth factor (PDGF) or basic fibroblast growth factor (bFGF). These results suggest that V-SMC change their phenotype in a competence-factor-dependent manner.
 ...
PMID:Competence growth factors evoke the phenotypic transition of arterial smooth muscle cells. 217 12

The Smc5-Smc6 complex contains a heterodimeric core of two SMC proteins and non-Smc elements (Nse1-6), and plays an important role in DNA repair. We investigated the functional roles of Nse4 and Nse1 in Dictyostelium discoideum. Nse4 and Nse3 expressed as Flag-tagged fusion proteins were highly enriched in nuclei, while Nse1 was localized in whole cells. Using yeast two-hybrid assays, only the interaction between Nse3 and Nse1 was detected among the combinations. However, all of the interactions among these three proteins were recognized by co-immunoprecipitation assay using cell lysates prepared from the cells expressing green fluorescent protein (GFP)- or Flag-tagged fusion proteins. GFP-tagged Nse1, which localized in whole cells, was translocated to nuclei when co-expressed with Flag-tagged Nse3 or Nse4. RNAi-mediated Nse1 and Nse4 knockdown cells (Nse1 KD and Nse4 KD cells) were generated and found to be more sensitive to UV-induced cell death than control cells. Upon starvation, Nse1 and Nse4 KD cells had increases in the number of smaller fruiting bodies that formed on non-nutrient agar plates or aggregates that formed under submerged culture. We found a reduction in the mRNA level of pdsA, in vegetative and 8 h-starved Nse4 KD cells, and pdsA knockdown cells displayed effects similar to Nse4 KD cells. Our results suggest that Nse4 and Nse1 are involved in not only the cellular DNA damage response but also cellular development in D. discoideum.
 ...
PMID:Nse1 and Nse4, subunits of the Smc5-Smc6 complex, are involved in Dictyostelium development upon starvation. 2603 68