Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0036690 (
sepsis
)
59,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Sepsis
is a critical, complex medical condition, and the major causative pathogens of
sepsis
are both
Staphylococcus aureus
(
S. aureus
) and
Escherichia coli
(
E. coli
). Genome-wide studies identify differentially expressed genes for
sepsis
. However, the results for the identification of DEGs are inconsistent or discrepant among different studies because of heterogeneity of specimen sources, various data processing methods, or different backgrounds of the samples. To identify potential transcriptional biomarkers that are differently expressed in
S. aureus
- and
E. coli
-induced
sepsis
, we have analyzed four microarray datasets from GEO database and integrated results with bioinformatics tools. 42 and 54 DEGs were identified in both
S. aureus
and
E. coli
samples from any three different arrays, respectively. Hierarchical clustering revealed dramatic differences between control and
sepsis
samples. GO functional annotations suggested that DEGs in the
S. aureus
group were mainly involved in the responses of both defense and immune regulation, but DEGs in the
E. coli
group were mainly related to the regulation of endopeptidase activity involved in the apoptotic signaling pathway. Although KEGG showed inflammatory bowel disease in the E. coli group, the KEGG pathway analysis showed that these DEGs were mainly involved in the tumor necrosis factor signaling pathway, fructose metabolism, and mannose metabolism in both
S. aureus
- and
E. coli
-induced
sepsis
. Eight common genes were identified between
sepsis
patients with either
S. aureus
or
E. coli
infection and controls in this study. All the candidate genes were further validated to be differentially expressed by an ex-vivo human blood model, and the relative expression of these genes was performed by qPCR. The qPCR results suggest that GK and PFKFB3 might contribute to the progression of
S. aureus
-induced
sepsis
, and CEACAM1, TNFAIP6,
PSTPIP2
, SOCS3, and IL18RAP might be closely linked with
E. coli
-induced
sepsis
. These results provide new viewpoints for the pathogenesis of both
sepsis
and pathogen identification.
...
PMID:Identification of Potential Transcriptional Biomarkers Differently Expressed in Both
S. aureus
- and
E. coli
-Induced Sepsis via Integrated Analysis. 3109 95
