Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Enzyme
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Query: UMLS:C0036690 (
sepsis
)
59,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Our previous data demonstrated that
Friend leukemia virus integration 1
(
Fli-1
), an ETS transcription factor, governs pericyte loss and vascular dysfunction in cecal ligation and puncture-induced murine
sepsis
by regulating essential pyroptosis markers including caspase-1. However, whether
Fli-1
regulates caspase-1 expression levels in vitro and how
Fli-1
regulates caspase-1 remain unknown. Our present work further demonstrated that overexpressed
Fli-1
significantly increased caspase-1 and IL-18 expression levels in cultured mouse lung pericytes. Bacterial outer membrane vesicles (OMVs) have been found to induce cell pyroptosis through transferring LPS intracellularly. Using OMVs to induce an in vitro model of pyroptosis, we observed that OMVs significantly increased protein levels of
Fli-1
in mouse lung pericytes. Furthermore, knockdown of
Fli-1
by siRNA blocked OMVs-induced caspase-1, caspase-11 and IL-18 expression levels. As caspase-1 was predicted as a potential target of
Fli-1
, we cloned murine caspase-1 promoter into a luciferase construct. Our data demonstrate for the first time that
Fli-1
regulates caspase-1 expression by directly binding to its promoter regions measured by chromatin immunoprecipitation (ChIP) assay and luciferase reporter system. In summary, our findings demonstrated a novel role and mechanism of
Fli-1
in regulating caspase-1 expression in lung pericytes.
...
PMID:Fli-1 transcription factor regulates the expression of caspase-1 in lung pericytes. 3073 75
