Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0036572 (seizures)
 80,221 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. During pentylenetetrazol-induced bursting activity which is characteristic intracellular potential change of seizure discharge, intracellular stored calcium is released and moved toward the inner surface of the cell membrane. Calcium is released from lysosome-like granules with morphological changes. During bursting activity, the intracellular free calcium level was higher than the normal state. During bursting activity, intracellular protein of 5 kDa and 15 kDa was changed qualitatively and quantitatively. 2. Primary cultured cerebral cortical neurons of rats and mice showed spontaneous regular firing, and by PTZ application, showed bursting activity. A single potassium channel showed the random open-close state in the normal state and showed burst type open-close state after PTZ application. 3. Primary cultured cerebral cortical neurons of the E1 mouse, the epilepsy animal model, showed developmental defects in neurite extension and content of gangliosides, in addition to their very high susceptibility to convulsions. 4. A new antiepileptic drug, TJ-960, which originates from a mixture of nine herbal drugs, normalized the above-mentioned seizure-related, calcium-related intracellular pathological phenomena. TJ-960 normalized cytochalasin-B-induced looping phenomena and protected the neuron damage induced by cytochalasin B in addition to anticonvulsant action. TJ-960 also completely normalized the cobalt-induced EEG changes and also protected against neuron damage in the hippocampus induced by cobalt application to the cerebral cortex. TJ-960 normalized the developmental defects of the E1 mouse neuron. 5. For better therapy of epilepsy, it is probably necessary to normalize the developmental defects and to protect against neuron damage in addition to inhibition of seizure activity.
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PMID:Cellular physiology of epileptogenic phenomena and its application to therapy against intractable epilepsy. 167 17

The comparative measuring of the dust-seizure-efficiency of aspirators is being carried out by a practice-related treatment of 5 cylindrical-shaped specimen made of a commercial cobalt-chromium-alloy with aluminum-oxide-grinding instruments and silicone-rubber-polishers. A small-filter-apparatus takes up the person related samplings of the occurring total-dust-amount, which is then being calculated by a gravimetric standard-measuring-procedure. The cobalt-chromium-dust-fraction in the total dust amount is determined through an x-ray-fluorescence-analysis. Workplace dust-aspiration-equipment of the firms KaVo, Freuding and Wassermann have been subjected to testing. The values of the efficiency of all aspirators turned out to be below the American Threshold Limit Value TLV-TWA (Threshold Limit Value-Time Weighted Average) of 0.5 mg/m3 for chromium. The strict TLV-TWA-value of 0.05 mg/m3 for cobalt it also--with the exception of the Freuding-aspirator A 84 with aspiration-tube--not exceeded.
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PMID:[Efficiency of various dental dust-aspiration-equipment regarding the treatment of cobalt-chromium alloys]. 181 57

Stereotactic radiosurgery successfully obliterates carefully selected arteriovenous malformations (AVM's) of the brain. In an initial 3-year experience using the 201-source cobalt-60 gamma knife at the University of Pittsburgh, 227 patients with AVM's were treated. Symptoms at presentation included prior hemorrhage in 143 patients (63%), headache in 104 (46%), and seizures in 70 (31%). Neurological deficits were present in 102 patients (45%). Prior surgical resection (resulting in subtotal removal) had been performed in 36 patients (16%). In 47 selected patients (21%), embolization procedures were performed in an attempt to reduce the AVM size prior to radiosurgery. The lesions were classified according to the Spetzler grading system: 64 (28%) were Grade VI (inoperable), 22 (10%) were Grade IV, 90 (40%) were Grade III, 43 (19%) were Grade II, and eight (4%) were Grade I. With the aid of computer imaging-integrated isodose plans for single-treatment irradiation, total coverage of the AVM nidus was possible in 216 patients (95%). The location and volume of the AVM were the most important factors for the selection of radiation dose. Magnetic resonance (MR) imaging was performed at 6-month intervals in 161 patients. Seventeen patients who had MR evidence of complete obliteration underwent angiography within 3 months of imaging: in 14 (82%) complete obliteration was confirmation being 4 months (mean 17 months) after radiosurgery. The 2-year obliteration rates according to volume were: all eight (100%) AVM's less than 1 cu cm; 22 (85%) of 26 AVM's of 1 to 4 cu cm; and seven (58%) of 12 AVM's greater than 4 cu cm. Magnetic resonance imaging revealed postirradiation changes in 38 (24%) of 161 patients at a mean interval of 10.2 months after radiosurgery; only 10 (26%) of those 38 patients were symptomatic. In the entire series, two patients developed permanent new neurological deficits believed to be treatment-related. Two patients died of repeat hemorrhage at 6 and 23 months after treatment during the latency interval prior to obliteration. Stereotactic radiosurgery is an important method to obliterate AVM's, especially those previously considered inoperable. Success and complication risks are related to the AVM location and the volume treated.
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PMID:Stereotactic radiosurgery for arteriovenous malformations of the brain. 158 16

To precisely evaluate a protein-related mechanism of epileptogenesis, we quantitatively analyzed the 70-kDa protein (namely, P70), a specific protein found in the cobalt-induced epileptic focus, and examined its effect on the electrocorticogram (ECoG) and cortical neurons in cerebral slices and its immunocytochemical localization in rats. Cobalt-induced cortical epileptogenic cortex exhibited a marked induction of P70. Its initiation time was ahead of the generation of epileptogenic activities. The anticonvulsant phenytoin (PHT) attenuated the cobalt-induced epileptogenic activities, but failed to suppress protein induction. Injection of this protein into the motor region of normal rat cerebral cortex elicited an epileptic ECoG and behavioral seizures. It also caused epileptiform activity with paroxysmal depolarization shifts in cortical neurons. These epileptogenic phenomena elicited by P70 were abolished by prior treatment with PHT or phenobarbital. Immunocytochemical analysis with an antiserum against P70 revealed that the reactivity was confined to pyramidal cells only in the region of the focus and was mainly localized on somatic, dendritic, and nuclear membranes and microtubles. These findings suggest that P70 may be linked to epileptogenesis.
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PMID:A novel protein induced in cortical epileptic focus of rat cerebrum: a possible linkage to epileptogenesis. 198 97

In cobalt-induced epileptogenic cortex in rats, a marked increase in two proteins of about 84 kDa and 70 kDa, a slight increase in 12-kDa and 10-kDa proteins and a decrease in a protein of about 57 kDa were noted, as determined by SDS-polyacrylamide gel electrophoresis. The initiation of these protein changes was ahead of the generation of epileptogenic activities. The anticonvulsant drugs phenytoin (PHT) and phenobarbital (PB) attenuated the cobalt-induced epileptogenic activities, but failed to suppress the protein changes. Among these proteins, a 70-kDa protein, when injected intracortically into the motor region of the normal rat cerebrum, evoked epileptic discharges on the electrocorticograph and behavioral seizure, which were abolished by prior treatment with PHT or PB. These findings suggest that the above protein changes are not an indirect offshoot of secondary stimulation of neurons by neurotransmitters or neuromodulators, and that P70 may contribute to the generation of epileptic seizure activities.
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PMID:Protein changes associated with cobalt-induced epileptic activity in rat cerebral cortex. 206 47

To evaluate the protein-related mechanism for epileptogenesis, we examined protein behavior during cobalt-induced epileptic seizure activity and the effect of injection of cobalt-induced proteins into cerebral cortex on electrocorticographic discharge in rats. Cobalt-induced epileptic seizure activity caused an increase in two proteins that migrated at about 84 kDa (P84) and 70-71 kDa (P70) and a decrease in a protein of about 57 kDa (P57) as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Among these proteins, P70 evoked epileptic discharges on the electroencorticograph and behavioral seizure, when it was intracortically injected into the motor region of the normal rat cerebrum. The data suggest that P70 may be a factor for epileptogenesis.
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PMID:Induction of epileptic seizure activity by a specific protein from cobalt-induced epileptogenic cortex of rats. 230 72

Review of 60 electroencephalograms recorded during episodes of generalized convulsive status epilepticus suggested that there are 5 identifiable EEG patterns which occur in a predictable sequence during the course of generalized convulsive status epilepticus in man: (1) discrete seizures; (2) merging seizures with waxing and waning amplitude and frequency of EEG rhythms; (3) continuous ictal activity; (4) continuous ictal activity punctuated by low voltage 'flat periods'; and (5) periodic epileptiform discharges on a 'flat' background. We confirmed our hypothesis that this sequence represents the natural history of electroencephalographic changes in untreated generalized convulsive status epilepticus by observing the same sequence in the EEGs of rats in which status epilepticus had been induced by 3 different methods: (1) systemic administration of kainic acid, (2) injection of homocysteine thiolactone to cobalt-lesioned rats; and (3) injection of lithium chloride followed 24 h later by injection of pilocarpine.
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PMID:A progressive sequence of electroencephalographic changes during generalized convulsive status epilepticus. 230 22

Acromegaly was diagnosed in 14 middle-aged to old cats of mixed breeding. Thirteen (93%) of the cats were male and one was female. The earliest clinical signs in the 14 cats included polyuria, polydipsia, polyphagia, all of which were associated with untreated diabetes mellitus. All developed severe insulin resistance within a few months; peak insulin dosages required to control severe hyperglycemia ranged from 20 to 130 U per day. Other clinical findings weeks to months after diagnosis included enlargement of one or more organs (e.g., liver, heart, kidneys, and tongue) (n = 14), cardiomyopathy (n = 13), increase in body size and weight gain (n = 8), nephropathy associated with azotemia and clinical signs of renal failure (n = 7), degenerative arthropathy (n = 6), and central nervous system signs (i.e., circling and seizures) caused by enlargement of the pituitary tumor (n = 2). The diagnosis of acromegaly was confirmed by demonstration of extremely high basal serum growth hormone concentrations (22 to 131 micrograms/l) in all cats. Computerized tomography disclosed a mass in the region of the pituitary gland and hypothalamus in five of the six cats in which it was performed. Two cats were treated by cobalt radiotherapy followed by administration of a somatostatin analogue (octreotide), whereas two cats were treated with octreotide alone. Treatment had little to no effect in decreasing serum GH concentrations in any of the cats. Eleven of the 14 cats were euthanized or died four to 42 months (median survival time, 20.5 months) after the onset of acromegaly because of renal failure (n = 2), congestive heart failure (n = 1), concomitant renal failure and congestive heart failure (n = 3), progressive neurologic signs (n = 2), persistent anorexia and lethargy of unknown cause (n = 1), the owner's unwillingness to treat the diabetes mellitus (n = 1), or unknown causes (n = 1). Results of necropsy examination in ten cats revealed a large pituitary acidophil adenoma (n = 10), marked left ventricular and septal hypertrophy (n = 7), dilated cardiomyopathy (n = 1), arthropathy affecting the shoulder, elbow, or stifle (n = 5), and glomerulopathy characterized by expansion of the mesangial matrix and variable periglomerular fibrosis (n = 10).
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PMID:Acromegaly in 14 cats. 240 66

Although the importance of neuronal synchrony in epilepsy has not been disputed, few attempts have been made to examine quantitatively the relationship between this parameter and seizure occurrence. The specific objective of the present investigation was to determine how the amount and type of synchrony between EEG and single-unit activity in an experimental model of focal epilepsy are related to the occurrence of seizures. This was accomplished by examining EEG/single unit relationships in two types of cobalt-induced epileptogenic focus: (1) foci that initiated seizures, and (2) foci that exhibited only interictal spike activity. These relationships were examined during slow-wave sleep, a time when synchronous neuronal activity is thought to be augmented. In control rats and rats that had seizures, the majority of units exhibited a non-random relationship between unit discharge and the EEG. In cobalt-treated rats that were not observed to have seizures, however, the percentage of units exhibiting EEG/single unit relationships was significantly less than that in either controls or rats that had seizures. This observation, paired with observations of the details of the EEG/single unit relationships, led to the hypothesis that cobalt treatment produces a shift from an inhibition dominated synchrony (observed in controls) to an excitation dominated synchrony (observed in rats that had seizures). Intermediate between these two types of synchrony is a less synchronized state (observed in seizure-free, cobalt-treated rats), which probably results from a loss of inhibition dominated synchrony without a concomitant increase in excitation dominated synchrony.
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PMID:Relationship between single-unit activity and the electroencephalogram in a neocortical, cobalt-induced epileptogenic focus. 244 19

In order to clarify its role in the provocation of seizure activity, the effects of cyclic AMP were examined on the intracellular calcium concentration of pentylenetetrazol (PTZ)-sensitive neurons as well as of PTZ-non-sensitive neurons of the Japanese land snail, Euhadra peliomphala. Extracellular application of isobutylmethylxanthine (IBMX) and dibutyryl cyclic AMP showed bursting activity-like firing in the PTZ-sensitive neurons. When 5'-guanylylimidodiphosphate (GNP-PNP) was injected into PTZ-sensitive neurons in the extracellular presence of IBMX and dibutyryl cyclic AMP, bursting activity followed by long-lasting hyperpolarization occurred. Intracellular injection of cyclic AMP into PTZ-sensitive neurons caused hyperpolarization coincident with an increase in intracellular calcium concentration. This increase in intracellular calcium concentration was the same under conditions in which the calcium influx was inhibited by the substitution of extracellular calcium chloride by cobalt chloride. In PTZ-non-sensitive neurons, cyclic AMP-induced bursting activity was not observed. These results suggest that an increase in cyclic AMP provoked bursting activity via an increase in intracellular calcium concentration.
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PMID:Action of cyclic AMP on intracellular calcium concentration and bursting activity. 246 57


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