Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0035412 (rhabdomyosarcoma)
 6,156 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have observed the features of malignant phenotypes in human rhabdomyosarcoma RD cells. These cells exhibited high growth rate, aberrant relation between cell proliferation and myogenic terminal differentiation, blockage of myofibrillogenesis, inhibition of gap junctional intercellular communication (GJIC) and inhibition of transcriptional expression of gap junction gene, connexin 43 (Cx43). By utilization of single cell subclone separation and cDNA cell transfection techniques, we obtained several RD subclones, among them 3 were representative of differences in phenotypes and were selected for further study. They were single-cell subclone RDL6, RDL3, and a Cx43 transfectant clone of RDL6, the RDL6/C-4. Through determination of cell growth doubling time, co-stain of 3H-TdR autoradiography with myosin heavy chain (MHC) immunofluorescence, immunofluorescent cytochemical examination and slot-blot analysis, it was demonstrated that significant differences existed between each of the 3 subclones. They were differnet in cell growth rate, cell proliferation and terminal differentiation coupling relation, progress of myogenesis, functional expression of Cx43 gene, and expression of proto-oncogenes, c-myc, Ha-ras and c-met products, therefore they could be used as cell models in the study of reverse transformation of rhabdomyosarcomas. The reversal differentiation markers, their examination approaches and practical value were discussed.
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PMID:[Studies on the cell models of differentiation reversal phenotypes of human rhabdomyosarcoma]. 758 89

Rhabdomyosarcoma (RD) cells express low levels of the gap junction protein connexin 43 (Cx43), and its mRNA, and display very weak gap junctional intercellular communication (GJIC) as detected by Cx43 immunofluorescence, slot-blot and dye-transfer methods. These cells grow rapidly and show aberrant and incomplete myogenic differentiation. To investigate the role of gap junctions in these cells, the expression of Cx43 with relation to cell growth and myogenic differentiation in RD single-cell subclones-RDL3 and RDL6 is studied. The subclone RDL3 grows slowly and displays better myogenic differentiation. The expression of Cx43, its mRNA and the GJIC in RDL3 is comparable to that in normal myoblasts. Another subclone RDL6 which grows rapidly, but is poorly differentiated, expresses very low levels of Cx43 and its mRNA, and very weak GJIC. By using the calcium phosphate precipitate transfection technique, a full-length cDNA-encoding Cx43 and a pSV2neo have been introduced into the RDL6 cells. Several stably transfected clones have been obtained. A stable Cx43-transfectant clone RDL6/C-4 expresses high level of Cx43 and its mRNA, and results in dramatic increase of GJIC. These cells grow slowly but display the enhanced myogenic differentiation. A correlation between the down-regulation of Cx43 gene expression and a reduced expression of myogenic differentiation in RD cells is is demonstrated. Forced expression of Cx43 not only inhibits cell growth but also correlates with the improved myogenic differentiation of RD cells.
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PMID:Functional expression of gap junction gene Cx43 and the myogenic differentiation of rhabdomyosarcoma cells. 776 12