Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0035412 (rhabdomyosarcoma)
 6,156 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

When leucocytes from normal young adults were cultured with BCG or tuberculine, in vitro, lymphoblasts were observed in the cultures. Such cultures, especially the cell free supernatant, when injected into 2 patients with recurrent thyroid carcinoma and rhabdomyosarcoma respectively, repeatedly caused necrosis of these tumour. Although the nature of the substance responsible for the necrosis is at present unknown, it was very potent, and non antigenic and probably of small molecular weight as it killed the tumour cells by diffusing to them in the tissue fluid. The patients who received the infusion were not cured of their tumours but the method holds promise for palliating and even possibly treating patients with advanced cancers not suitable for other forms of treatment. It could also be used in place of BCG vaccination in Hodgkins disease, leukaemias and other lymphoreticular tumours with a weakened or incompetent cell mediated immune system-CMIS. This study provides further support for recommending regular BCG vaccination of normal people as a prophylaxis against cancer.
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PMID:The immunobiotherapy of cancer: a preliminary report of a study of leucocytes cultured with B. C. G. or tuberculine on malignant disease. 1629 56

Development of multigenic constructs expressing Mycobacterium tuberculosis (Mtb) antigens may be a strategy to obtain improved DNA vaccines against tuberculosis (TB). Several multigenic constructs expressing two or three Mtb antigens as fusion proteins were developed, both as tPA- and ubiquitin-fusion proteins. To demonstrate proper protein expression and intracellular turnover all multiantigens were tagged with the HA epitope and constructs were used to transfect rhabdomyosarcoma (RD) cells. Antigen expression was demonstrated by immunofluorescence using anti-HA antibodies. C57Bl/6 mice were immunized with selected constructs and protective activity was assessed following aerogenic challenge with Mtb. Several of these constructs induced a significant level of protection in the lung and in the spleen. Immunization with the construct expressing tPA85B-ES6 induced a level of protection that approached that provided by BCG. Immunization with a combination of these constructs induced levels of protection that were not superior to those elicited by a single combination, and immunization with a construct expressing five Mtb antigens could not provide an improved level of protection compared to tPA85B-ES6. We conclude that the activity of a DNA vaccine based on tPA85B-ES6 cannot be enhanced by broadening the antigen repertoire with other highly immunogenic secreted Mtb proteins.
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PMID:Evaluation of the anti-tuberculosis activity generated by different multigene DNA vaccine constructs. 1846 35