Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0035412 (rhabdomyosarcoma)
 6,156 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The fused gag-v-myc oncogene was microinjected into fertilized mouse eggs, which were then implanted into foster mothers. Approximately 26% of the offsprings from injected eggs carried v-myc sequences. 26 of 32 progeny animals were found to be transgenic and some progeny containing the amplified oncogene (about 40 copies per genome). In one F0 and one F1 mice 1.5-2 months after birth the development of tumors was observed: rhabdomyosarcoma and sebaceous carcinoma. In both the cases the tumors were highly differentiated. Because spontaneous tumors of these types are seldom observed in common lines of mice it seems probable that the tumors observed in this study may be associated with the presence of oncogene v-myc.
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PMID:[Viral myc oncogene in the genome of transgenic mice and their progeny]. 284 17

The mobile transgene constructs of most human immunodeficiency virus (HIV)-based lentivirus vectors currently in use contain viral long terminal repeats, a 5' untranslated region, gag sequences, and env sequences that include the Rev-responsive element (RRE). In this study, we examined the possibility of deleting HIV splice sites and gag and env sequences from an HIV type 1 recombinant vector established in our laboratory as part of our ongoing efforts to improve this vector system. Mutations in the major splice donor site (SD) markedly reduced viral RNA expression but had little effect on vector titer. Deletion of gag or env sequences, excluding RRE, led to a moderate reduction in vector titer. Interestingly, deletion of RRE slightly reduced viral RNA expression but markedly impaired vector function. Combined deletions of RRE, gag (except for the first 40 nucleotides), env, and the SD mutation resulted in a twofold increase in cytoplasmic viral RNA expression and a recovery of vector efficiency to approximately 50% of the wild-type level. This increase in cytoplasmic RNA levels is likely to be due, at least in part, to effects of the TE671 host cells, a human rhabdomyosarcoma cell line used for vector production in our system, on the cytoplasmic distribution of spliced and unspliced viral RNA. These results show that optimal lentivirus vector function can be maintained in the absence of multiple essential viral elements.
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PMID:Contributions of viral splice sites and cis-regulatory elements to lentivirus vector function. 1036 78