Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0034069 (
pulmonary fibrosis
)
7,050
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This study aims to investigate the effects of
TRB3
on the EMT and MAPK signaling pathways in a bleomycin (BLM)-induced
pulmonary fibrosis
mouse model.
TRB3
adenovirus vector with green fluorescent protein (GFP) and
TRB3
-siRNA adenovirus vector were constructed for overexpression and down-regulation of
TRB3
, respectively. The
pulmonary fibrosis
mouse model was induced by bleomycin, and then treated with adenovirus on the next day. The mice were randomly killed at the 7
th
(D7), 14
th
(D14) and 28
th
(D28) day, respectively. The lung tissues were collected for histopathologic observations, hydroxyproline determination, Immunohistochemistry, western blot and RT-qPCR to detect the expression of
TRB3
and EMT-related proteins. Overexpression of
TRB3
caused more severe
pulmonary fibrosis
(P<0.05), while downregulation of
TRB3
significantly reduced
pulmonary fibrosis
(P<0.05). The expression of MAPK pathway-related and EMT-related genes and proteins was markedly upregulated by
TRB3
overexpression (P<0.05), but prominently downregulated by
TRB3
-shRNA (P<0.05). In conclusion, exogenous regulation of
TRB3
may have effects on bleomycin-induced
pulmonary fibrosis
in mice.
...
PMID:TRB3 regulates pulmonary interstitial fibrosis through the MAPK signaling pathway. 3193 68
