Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0033687 (proteinuria)
 24,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Rabbits immunized with ultraviolet-irradiated DNA (UV-DNA) produced high titers of serum antibody. This experimental model was studied to determine if injection of antigen (UV-DNA) intravenously into immunized animals would induce glomerulonephritis and proteinuria. Proteinuria was observed several days after the start of daily intravenous injections into immunized animals and was sustained as long as injections were continued, but fell to normal values after stopping antigen administration. The kidneys showed glomerulitis sometimes associated with focal proliferative lesions, and immunofluorescence showed rabbit Ig and C3 in glomeruli. By electron microscopy, electron-dense subendothelial deposits were seen. Sucrose density gradient analyses of sera immediately after antigen injections suggested the presence of immune complexes of DNA and antibody since both heavy sedimenting and 7S Ig were detected. After digestion with deoxyribonuclease rabbit Ig could be found only in the 7S sedimenting fractions. Intravenous injection of UV-DNA into normal, nonimmune animals did not produce heavy sedimenting Ig or abnormal sedimentation patterns. These studies with an experimental model might provide insight into pathogenetic mechanisms operating in systemic lupus erythematosus where the importance of DNA-anti-DNA immune complexes have been documented. The studies suggested that gradual accumulation of DNA immune complexes in glomeruli might be one mechanism causing renal functional abnormalities.
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PMID:Experimental renal disease induced by DNA-anti-DNA immune complexes. 455 Apr 91

Antibodies to single-stranded DNA (anti-ssDNA) and double-stranded DNA (anti-dsDNA) were determined in serum and urine of nine patients with systemic lupus erythematosus (SLE) presenting with heavy proteinuria (greater than 300 mg/dl), and the activity of anti-DNA antibodies was compared between paired serum and urine samples in each patient. The results were expressed by the anti-DNA activity (radioimmunoassay units per milliliter) divided by the concentration of IgG (milligrams per milliliter), so as to correct for the difference of IgG concentration. Anti-DNA was detected invariably in the serum of these patients (anti-ssDNA/IgG 4.8 to 27.2, anti-dsDNA 5.6 to 107.8). In contrast, anti-DNA activity was not detectable in urine samples from any of the nine patients. The urine samples from these patients, as well as those from normal individuals, were found to contain ssDNA at levels not significantly different from each other (0.25 +/- 0.15 vs. 0.19 +/- 0.07 microgram/mg creatinine, P greater than 0.1). The failure to detect anti-DNA in the urine of SLE patients, however, was not due to the ssDNA contained in the urine, because no anti-DNA activity was detected even after their urine samples had been digested with deoxyribonuclease. On the basis of these results, anti-DNA in the serum of SLE patients was considered to be entrapped in the kidney, probably owing to its binding with DNA deposited in their glomeruli.
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PMID:Entrapment of anti-DNA antibodies in the kidney of patients with systemic lupus erythematosus. 698 73