Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0033687 (proteinuria)
 24,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A study group of 623 employed Swiss women aged 30-49 years showing objective evidence of intake of phenacetin-containing analgesics, and a control group of 621 comparable women showing no such intake, were observed for 4 years (1969-72) for laboratory evidence of urorenal disorders. In both study and control groups morbidity was low. There was no difference between the study and control groups with respect to subsequent proteinuria, bacteriuria, and haematuria. The 4-year incidence of low urine specific gravity after overhight thirsting was significantly higher in the study group than in the control group (3-8% v. 0-8%) and the incidence of raised serum-creatinine was also significantly higher in the study group (2-9% v. 0-4%). However, when the study group was further subdivided into a sub-group showing evidence of high intake of phenacetincontaining analgesics and one showing low intake, only the high-intake subgroup had an incidence of raised serum-creatinine (5-4%) significantly higher than the control group (0-4%), whereas the low-intake subgroup had an incidence (0-4%) similar to the control group.
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PMID:Relation between regular intake of phenacetin-containing analgesics and laboratory evidence for urorenal disorders in a working female population of Switzerland. 4 16

A patient with paroxysmal myoglobinuria presented with low molecular weight (LMW) proteinuria in association with an episode of exertional myoglobinuria. Since no signs of acute renal failure were present, the cause was probably competition between myoglobin and other LMW proteins for proximal tubular reabsorption. Agarose gel electrophoresis was found to be an excellent method for the investigation of myoglobinuria since this technique not only allowed the separation of myoglobin from hemoglobin but also myoglobin from metmyoglobin.
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PMID:Low molecular weight proteinuria in association with paroxysmal myoglobinuria. 4 77

Normal rats were injected with guinea pig anti-rat glomerular basement membrane antibodies of the IgG1 or IgG2 class or with their F (ab') 2 fragments, in order to study which antibody site triggers the alternate complement pathway in vivo. Both IgG classes were able to induce a heavy proteinuria and led to C3 deposition in the glomeruli in a pattern similar to their own distribution along the glomerular basement membrane, as shown by the immunofluorescence technique. The Fab(ab')2 fragment of IgG2 did not produce C3 binding or proteinuria. The F(ab')2 fragment of IgG1 was difficult to obtain devoid of Fc determinants. A F(ab')2 fragment of IgG1 still bearing Fc determinants led to C3 binding and proteinuria, whereas the true F(ab')2 fragment of IgG1 had none of these effects in two out of three animals.
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PMID:Further studies on the biologic properties of guinea pig IgG1 antibodies. II. In vivo activation of C3 by anti-glomerular basement membrane antibodies. 5 Mar 74

The electrophoretic study of tubular proteinuria, carried out on the acrylamide-agarose electrophoresis, shows various patterns of tubular proteinuria characterized by low molecular weight proteins: alpha2-microglobulin, beta2-microglobulin and post gamma. The immunoprotidologic study of the microglobulins is difficult since they have a similar molecular weight. The zonal acrylamide-agarose electrophoresis allows the isolation of beta2m and post gamma. Recent studies suggest a number of hypothesis on the function of the microglobulins.
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PMID:[Tubular proteinuria (electrophoretic studies and properties of the microglobulins)]. 5 Jun 7

In a survey of the qualitative methods of research into proteinuria, the Authors recall, apart from later modifications, the old techniques based on the occurrence of turbidity from precipitation of proteins obtained with heat and/or by the use of various anionic reagents. Particular attention is given to the rapid tests with paper strip based on the phenomenon of error made by indicators in the presence of protein solutions. Their sensitivity, their specificity and above all the fact that their use is unbeatably practical are discussed, both on the basis of data derived from other studies and on the basis of various personal observations. From the latter, the Authors deduce and demonstrate the substantial constancy in the behaviour of various types of paper strip test, even those produced in different periods and by different manufacturers; how the data obtained with these various papers can be compared with the best modern methods of measuring turbidity; and the different sensitivities of the papers with various types of proteins such as albumins, globulins and in particular immunoglobulinic micromolecular fragments. Finally, they show that falsely positive reactions in alkaline urines because of ammoniacal fermentation cannot be ascribed to the increase of pH, but rather directly to an excess of ammonium ions. After having remarked that tests with paper strip have to a large extent taken the place of classical methods, more for obvious reasons of expediency than for any real qualitative superiority, the Authors maintain that it is advisable, though only in border-line cases, to make a routine check with one of the traditional methods in order to screen genuine cases of proteinuria.
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PMID:[Methods of qualitative detection of proteinuria in mass surveys]. 5 Jun 8

In unconcentrated specimens measurements have been made of total proteins, by DOETSCH gel-filtration method, and of albumin, transferrin and of alpha-2-macroglobulin, by LAURELL electroimmunodiffusion technique. The results confirm that the gel-filtration method provides specific, reliable measurement of proteins in amount as little as 50 mg/1. The proteinuria of 21 samples of urine has been determined by the DOETSCH method and by the biuret method after protein precipitation by perchloric, trichloracetic and phosphotungstic acid. Only the use of phosphotungstic acid as precipitant provides a good correlation of results between the biuret method and the direct gel-filtration-biuret method. LAURELL electroimmunodiffusion technique was improved to allow the determination of 5 mg/1 of albumin, and transferrin, and of 1 mg/1 of alpha-2-macroglobulin. The AA. emphasize the advantages of using sensitive methods which do not require the preliminary concentration of the sample, for the analysis of urinary proteins.
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PMID:[Determination of urinary total proteins and some protein fractions]. 5 Jun 9

Solitary myeloma of bone occurred in 10 Black patients during the 8-year period from 1967 to 1974. In 4 patients, the solitary myeloma involved the bones of the paranasal sinuses, in 3 patients the pelvis, and in 3 others the manubrium sterni. An IgG monoclonal gammopathy was present in the serum of 4 patients and Bence-Joanes proteinuria was found in 1 patient. Radiation therapy was the treatment of choice and the recommended tumour dosage is 4000-5000 rads. Serial measurements of serum and urine protein electrophoresis and immuno-electrophoresis were most helpful in determining when patients had achieved a complete remission. In 3 patients, 1 of whom died, the myeloma disseminated in periods varying from 26 months to 7 years. Nine patients are alive, of whom 7 are in complete remission, and 5 have lived for more than 5 years since the initial diagnosis of solitary myeloma.
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PMID:Solitary myeloma. A study of 10 black patients during an 8-year period. 5 Jun 28

Using chromatographic technique on "Sephadex G 200" associated to immunologic qualitative and quantitative techniques, the authors showed that the alpha1-macroglobulin is present in normal Rat urines. This protein is also present in urines obtained during the Masugi nephritis. The alpha1-macroglobulin concentration observed in the different urine samples is unrelated to the total proteinuria evolution. The results given allow us to discuss the mechanisms of the glomerular filtration.
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PMID:[Changes in the level of alpha1-macroglobulin in rats during Masugi nephritis]. 5 Aug 89

Cells from human glomeruli explanted in tissue culture were grown and subcultivated up to 12 to 13 times. Light and electron microscopic studies revealed these cells to be morphologically distinct from fibroblasts. By electron microscopy, an extracellular material resembling basal lamina was seen and prominent intracellular microfilaments were evident. Immunofluorescent microscopy demonstrated reactivity of heterologous antiglomerular basement membrane antibody with aggregates of extracellular material. Absorption experiments using antiglomerular basement membrane antibody showed that the extracellular materiial shared some antigenic components with glomerular basement membrane. Antibody to cultured glomerular cells stained the mesangium and glomerular basement membrane of normal human kidney. This antibody was nephrotoxic in monkeys, induced proteinuria with proliferative glomerulonephritis, and localized to the mesangium and glomerular basement membrane of monkey glomeruli. These findings and the presence of prominent intracellular microfilaments (contractile elements) suggest that the glomerular cells may be of mesangial origin.
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PMID:Human glomerular cells in tissue culture. 5 Nov 34

Although the precise etiologic incitant of the minimal lesion idiopathic nephrotic syndrome of childhood is not known, it is likely that a host mechanism mediates the permeability alterations of the glomerular capillary wall resulting in massive proteinuria. As a first step in examining the possibility that local kinin release may account for the proteinuria in this disorder, two parameters of the plasma kinin-generating system, plasma prekallikrein and kallikrein inhibitor, were assayed during 27 nephrotic episodes in 21 corticosteroid-responsive children. Plasma kallikrein was assayed by means of its esterase activity on a synthetic arginine ester substrate, N-alpha-tosyl-L-arginine methyl ester (TAMe), after activation of Hageman factor by kaolin. This activity, after subtraction of spontaneous arginine esterase activity (i.e., TAMe esterase activity measured in plasma not exposed to kaolin) is derived from prekallikrein. Plasma prekallikrein activity in 11 normal children was 99.6 +/- 2.9 mumol TAMe hydrolyzed/ml plasma/hr (mean +/- SEM). Kallikrein inhibitor was quantified in arbitrary units. Kallifrein inhibitor activity in 11 normal children was 0.94 +/- 0.04 units. During the overt nephrotic syndrome, before initiation of intensive daily corticosteroid treatment, mean values were: prekallikrein, 58.5 +/- 7.24 mumol/ml/hr; and kallikrein inhibitor, 0.35 +/- 0.06 units. After corticosteroid-induced remission occurred, mean values were: plasma prekallikrein, 118.6 +/- 3.2 mumol/ml/hr; and kallikrein inhitor, 0.78 +/- 0.03 mumol/ml/hr. Both parameters were again assayed in 14 of the 21 children after complete cessation of corticosteroid treatment. Plasma prekallikrein was normal, 99.6 +/- 4.8 mumol/ml/hr; but kallikrein inhibitor was still somewhat depressed, 0.84 +/- 0.03 units. A subset of 9 patients had marked depression of plasma prekallikrein to levels less than 20 mumol/ml/hr and essentially undetectable inhibitor activity. Serum alpha-2 macroglobulin was elevated in nephrotic patients: mean value during relapse, 862 +/- 29 mg/100 ml; during corticosteroid-maintaining remission, 615 +/- 29 mg/100 ml. After cessation of corticosteroids, mean serum level was 481 +/- 20 mg/100 ml. The proportional reduction of plasma prekallikrein and kallikrein inhibitor suggested that an enzyme-inhibitor complex formed in vivo, perhaps at a local site of activation in proximity to the glomerular basement membrane. These data suggest that the plasma kinin-generating system may be the host effector mechanism subserving the increased glomerular capillary permeability in the minimal lesion nephrotic syndrome of childhood.
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PMID:A study of the plasma kinin-generating system in children with the minimal lesion, idiopathic nephrotic syndrome. 5 8


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