Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0033036 (APC)
 10,214 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enriching for a rare cell population with a combination of monoclonal antibodies (MAbs). Such an isolation scheme involves multi-step procedures including ficoll-density fractionation and presort enrichment followed by cell sorting. Over the past decade, various cell-surface and metabolic markers have been identified and used to isolate human HSCs and progenitors as summarized in Table 1. Among them, CD34 has become the most critical cell-surface marker for positively selecting a rare cell population (1,2). Within the CD34(+) cell population, the differential expression of Thy-1, CD38, and AC133 have been used to fractionate HSCs and progenitors. In order to subfractionate CD34(+) cells by these markers, the cells can be further purified by flow cytometry. HSCs can be further enriched into a Thy-1(+) (3-7), CD38(-lo) (8-10), Thy-1+ CD38(-lo) (11), or AC133+ (12,13) fraction of CD34(+) cells. Table 1 Commercially Available Cell-Surface and Metabolic Markers for Isolation of Human HSC and Progenitor Cells Marker Expression/Remark Fluorochrome conjugate recommended Reference Positive marker CD34 Positive FITC, PE, APC, BIO 1,2,33 Thy-1 Positive PE, BIO 3,4 AC133 Positive PE 12,34 Negative/low marker CD38 Negative /low FITC, PE, APC 8,9 HLA-DR( a ) Negative to low FITC, PE 35,36 Mature lineage marker, Lin- CD2 T-cell lineage FITC, PE, BIO 3 CD3 T-cell lineage FITC, PE, APC, BIO 3 CD19 B-cell lineage FITC, PE, APC, BIO 3 CD16 NK-cell lineage FITC, PE, APC, BIO 3 CD14 Myeloid lineage FITC, PE 3 CD15 Myeloid lineage FITC, PE 3 Glycophorin A Erythroid lineage FITC, PE 3 2nd Step reagent Avidin/Streptavidin For BIO MAb FITC, PE, APC, TXRD, PharRed, Cy-chrome( d ) Metabolic marker( b ) Rhodamine 123( c ) Low Mitochondria-binding dye 37,38 Hoechst 33342( c ) Low DNA-binding dye 39,40 Pyronin Y Low RNA-binding dye 39,40 Propidium iodide Negative to low Dead-cell exclusion Abbreviations: FBM, fetal bone marrow; MPB, mobilized peripheral blood; ABM, adult bone marrow; HSC, hematopoietic stem cells; FITC, fluorescein; PE, phycoerythrin; APC, allophycocyanin; TXRD, Texas red; BIO, biotinylated. ( a ) FBM, MPB HSCs express HLA-DR (41,42). ( b ) To isolate quiescent HSC. ( c ) Substrates for p-glycoprotein, encoded by MDR-1. HSC possess high levels of p-glycoprotein efflux activity. ( d ) Recommended for single laser flow cytometry only, lineage marker positive and PI positive cells can be excluded simultaneously.
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PMID:Method for purification of human hematopoietic stem cells by flow cytometry. 2143

We explored the role of APC-mediated MDR-1/CLCX-1 signaling pathway in ovarian tumors. In this study, ovarian tumor cell lines SKOV-3, ES-2 and MCV-152 were used to conduct the research. Fluorescence quantitative PCR and Western-blotting were used to investigate the effects of MDR-1/CLCX-1 signaling pathway in ovarian tumors. The effects of the APC gene silencing and overexpression on proliferation and apoptosis of ovarian tumor cells were detected by flow cytometry. Compared to normal cells, the expression of APC gene mRNA in ovarian tumor cells was significantly decreased (p less than 0.05). Western-blotting results showed that the level of APC protein in ovarian tumor cells was significantly lower than that in normal tissue, while MDR-1/CLCX-1 related proteins levels were significantly increased (p less than 0.05). In the APC gene silenced ovarian tumor cell lines, the expression of MDR-1/CLCX-1 was significantly higher than that of the untreated group (p less than 0.05), and apoptosis of ovarian tumor cells decreased. However, in ovarian tumor cell lines that over-expressed APC gene, the expression of MDR-1/CLCX-1 was significantly lower than that of the untreated group (p less than 0.05), and apoptosis of ovarian tumor cells was increased. There is a certain correlation between the APC gene and ovarian tumors, and the APC gene mediates the apoptosis of tumor cells through the MDR-1/CLCX-1 signaling pathway.
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PMID:Role of APC-mediated MDR-1/CLCX-1 signaling pathway in ovarian tumors. 2992 77