Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0032463 (polycythemia vera)
 3,374 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The methylation state of CCGG sites in and around the human ornithine decarboxylase gene, oncogenes c-myc and erb-A1, and actin genes were determined in human malignant leucocytes from patients with acute and chronic myeloid leukemia, chronic lymphatic leukemia, polycythemia vera, and multiple myeloma by means of isoschizomeric restriction endonuclease analysis. When compared with DNA from leucocytes of healthy controls, the ornithine decarboxylase and erb-A1 genes were substantially hypomethylated in all samples obtained from patients with chronic lymphatic leukemia. Hypomethylation of genes, particularly growth-related sequences, might be a crucial fact in the malignant transformation of human leucocytes. Its relatively simple detection from blood samples may prove clinically applicable in monitoring patients with chronic lymphatic leukemia.
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PMID:Hypomethylation of ornithine decarboxylase gene and erb-A1 oncogene in human chronic lymphatic leukemia. 290 92

On the basis of cloning and indentifying gE-gI gene of pseudurabie virus SH strain, the transfer plasmid vector was constucted in order to get the gE-gI gene partial deletion mutant. At first, gE gene and gI gene were cloned into pUC18, constructed the pgEI vector. Then, the 5' terminal sequence of gE gene was deleted 363bp using the restrict endonuclease in gE gene. The GFP expressing cassette was inserted into the deleting site. The recombinant plasmid pgEI including GFP reporter gene deleted part of gE-gI gene was constructed. BHK-21 cell which was infected with PRV-SH for 1-2h were tansfected with the complex of pgEI-GFP and DOTAPA deletion mutant was selected and purified many times in BHK-21 cell through GFP. Inoculation of mice with 2.0X107 PFU of the recombinant virus revealed that mice were partly protected against challenge with PRV-SH containing 2MLD.
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PMID:[Construction of pseudorabies virus SH strain with gE-gI gene partial deletion mutant including GFP reporter gene]. 1627 66