Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0032285 (pneumonia)
 54,520 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The lungs of 334 pigs were obtained from two slaughter plants in Minnesota and examined in detail. Macroscopic and microscopic evaluation, direct fluorescence for Mycoplasma hyopneumoniae and bacterial culture were done on all of them and a subsample of 50 were selected for virus culture. Mycoplasma hyopneumoniae, Pasteurella multocida and Haemophilus spp. were detected in 24.0%, 34.1% and 27.0% of the lungs, commonly in conjunction with each other. One isolate of Haemophilus pleuropneumoniae serotype 2 was detected and this represents the first report of its presence in the United States. No virus was detected in any of the lungs. Lungs with both M. hyopneumoniae and Pasteurella multocida had the greatest amount of macroscopic pneumonia (9.8% of the lung). Lungs with M. hyopneumoniae or P. multocida alone had 4.9% and 5.2% of the lung involved with pneumonia respectively. Lungs with Haemophilus sp. Taxon "minor group" had 3.8% of the lung involved which was not significantly different from lungs with none of these organisms being detected (1.6%). There was a positive correlation between the extent of M. hyopneumoniae infection, as scored by FAT and the amount of macroscopic pneumonia present (r = 0.46; P less than 0.001). Likewise, there was a positive correlation between the estimated concentration of P. multocida present, as scored by the relative number of colonies on blood agar and the amount of macroscopic pneumonia present (r = 0.60; P less than 0.001). Microscopically, the amount of lymphoreticular proliferation, polymorphonuclear cells and alveolar macrophages were evaluated.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Microorganisms associated with pneumonia in slaughter weight swine. 401 78

This study was undertaken to investigate the role of parainfluenza virus 3 (PIV3) in respiratory infection of camels. A total of 273 lung specimens from camels with pneumonia lesions were collected from slaughterhouses in four different areas of Sudan. In addition, eight specimens were collected from outbreaks of respiratory infection in camels. Using antigen detection sandwich ELISA kits, six out of the 281 specimens tested were positive for the PIV3 antigen (2.1%); the highest prevalence was noted in Eastern Sudan (4.2%), then in Central and Northern Sudan (1.4%). The direct immunofluorescent test (FAT) was used to confirm the positive reactions for PIV3 by ELISA. The polymerase chain reaction (RT-PCR) was applied for the detection of the PIV3 genome in lungs of camels; two out of four samples which were positive by the PIV3 ELISA were also positive by RT-PCR. Virus isolation was attempted for PIV3 in MDBK cells; four specimens yielded cytopathic virus when inoculated onto the cell culture. The cytopathic effect (CPE) consisted of cell rounding, multinucleated cells, sloughing and elongation of cells, and some syncytia were observed on the 3rd to 7th day post-inoculation. Using commercially available indirect ELISA kits for antibodies to PIV3, 495 camel sera were tested, and the seroprevalence detected was 82.2%. The highest seroprevalence was observed in Central (92.6%), then in Eastern (92.2%) and Central to South Sudan (82.5%); the lowest prevalence was found in Northern Sudan (64.8%).
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PMID:Respiratory infection of camels associated with parainfluenza virus 3 in Sudan. 1973 93

This study aimed to investigate the occurrence of respiratory syncytial virus (RSV) infections in camels in Sudan. A total of 272 camel lung specimens showing pneumonia were collected from slaughter houses at four different areas in Sudan, additionally 8 specimens were collected from outbreaks of respiratory infection in camels. Using sandwich ELISA kits for RSV antigen detection 4 out of 280 tested lungs (1.4%) were positive, all were from Central Sudan (Tambool slaughter house). FAT was used to confirm the ELISA positives. Polymerase chain reaction RT/PCR was applied for the detection of RSV genome in camel lungs; 1 out of 4 ELISA positives was positive by RT/PCR. Using indirect ELISA kits 135 out of 495 (27.3%) camel sera showed antibodies to RSV, highest prevalence was observed in Western (33.5%) then Central (31.6%) and Eastern Sudan (23.5%). Based on the manufacturer specified calculations for OD readings, most of positive sera (90/135) were low reactive (1+). This is the first report for the detection of RSV antigen, genome and antibody in camels in Sudan.
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PMID:Respiratory syncytial virus infection of camels (Camelus dromedaries). 1984 Jul 69

Pneumonia in bovines is a multifactorial disease manifestation leading to heavy economic losses. Infections of bovine respiratory syncytial virus (BRSV) and bovine parainfluenza virus-3 (BPI-3) are among the important contributing factors for the development of pneumonia in young animals. These viral agents either primarily cause pneumonia or predispose animals to the development of pneumonia. Although, the role of BRSV and BPI-3 in the pathogenesis of pneumonia is well established, there are no reports of involvement of BRSV and BPI-3 from Indian cattle and buffaloes suffering from pneumonia. In the present investigation, we performed postmortem examinations of 406 cattle and buffaloes, which were below twelve months of age. Out of 406 cases, twelve (2.95%) cases were positive for BRSV and fifteen (3.69%) cases were positive for BPI-3, screened by reverse transcriptase polymerase chain reaction (RT-PCR). Further, positive cases were confirmed by sequence analysis of RT-PCR amplicons and direct immunofluorescence antibody test (d-FAT) in paraffin-embedded lung tissue sections. BRSV positive cases revealed characteristic findings of bronchiolar epithelial necrosis, thickened alveolar septa by mononuclear cells infiltration and edema; alveolar lumens were filled with mononuclear cells and numerous syncytial cells were seen having intracytoplasmic inclusions. The BRSV antigen distribution was found to be in bronchiolar and alveolar epithelium and syncytial cells in the lung sections. In fifteen cases, where BPI-3 was detected, bronchointerstitial pneumonia in the majority of cases with thickened alveolar septa by mild macrophage infiltration, hyperplasia of type-II pneumocytes and bronchiolar necrosis along with syncytial cells having intracytoplasmic inclusions in the majority of cases were observed. The BPI-3 antigen distribution was found to be in bronchiolar and alveolar epithelium and syncytial cells in the lung sections. RT-PCR amplicons of BRSV and BPI-3 obtained were sequenced and their analysis showed homology with already available sequences in the NCBI database. It is the first report of detection of BRSV and BPI-3 from pneumonic cases by RT-PCR and d-FAT from cattle and buffaloes of India, indicating the need for more epidemiological studies.
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PMID:Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India. 3220 8