Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0031099 (periodontitis)
 12,489 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Little is known about the presence of common medical pathogens in the human oral cavity. Using a 16S rRNA-based PCR identification method, this study determined the occurrence of Porphyromonas asaccharolytica, Bacteroides fragilis and Chlamydia pneumoniae in subgingival plaque from 50 adults with advanced periodontitis. Each patient contributed samples from 3 deep periodontal pockets collected by paper points. The PCR primers were for P. asaccharolytica 5'-CTC TAG CTA GAG TGT ACT GG-3' and 5'-ATA GGG TTT ATA GAT TAG CTC TCT-3', for B. fragilis 5'-AAT GAT TCC GCA TGG TTT CAT TA-3' and 5'-GCG GTG ATT GCT CAC TGA CA-3', and for C. pneumoniae 5'- TGA CAA CTG TAG AAA TAC AGC-3' and 5'-CGC CTC TCT CCT ATA AAT-3'. The primers yielded a single amplicon with the respective reference strains and produced no amplicon with colonies of 25 groups of oral organisms. None of the three test species were detected in any of the 50 pooled subgingival samples tested. P. asaccharyolytica, B. fragilis and C. pneumoniae do not seem to be part of the periodontopathic microbiota in humans.
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PMID:Absence of Porphyromonas asaccharolytica, Bacteroides fragilis and Chlamydia pneumoniae in human subgingival plaque. 957 14

Smoking is detrimental to periodontal tissues, and periodontal destruction is greater among smokers. Paradoxically, smokers seem to have less gingival bleeding than never-smokers with comparable supragingival plaque. There is scarce information about the impact of smoking on gingival crevicular fluid (GCF) volume. This single-arm study clinical trial assessed the effect of smoking on GCF volume during the treatment of gingivitis. The sample included 24 never-smokers (47.3 +/-6.7years old, 41.7% males) and 21 smokers (45.8 +/- 5.1 years old; 55% males; 19.6 +/- 11.8 cigarettes/day; 24.1 +/- 8.7 years of smoking) with gingivitis and chronic periodontitis. After baseline supragingival scaling, patients received oral hygiene instructions weekly for 180 days. Particqants were examined at baseline, 30, 90 and 180 days, and gingival bleeding index (GBI), bleeding on prob-ing (BOP), periodontal probing depth (PPD) and GCF volume were recorded. Statistical analysis was performed using linear models (Wald test, p<0.05%). Smokers had significantly smaller GCF volumes than never-smokers. This finding was not attributed to GBI, BOP or PPD. Higher volumes of GCF were significantly associated with deeper pockets. GCF was significantly reduced throughout the study for both smokers and never-smokers, and the largest reductions were seen at 30 days. Smoking affected the GCF crevicular fluid volume independently of the presence of gingival bleeding, BOP and PPD. Smoking status and PPD should be taken into account when GCG volume and components are under investigation.
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PMID:The effect of smoking on gingival crevicular fluid volume during the treatment of gingivitis. 2030 19

Toll-like receptors (TLRs) contribute to the immune response by recognizing patterns presented by bacteria and other pathogens. These receptors have been implicated in the inflammatory response that contributes to gingivitis and periodontitis. Conflicting reports have suggested that variations in the genes encoding TLRs, particularly TLR2 and TLR4, may influence susceptibility to periodontitis. In this study, the contribution of variations in the genes encoding TLR2 and TLR4 in the context of periodontitis was examined in 254 patients with moderate periodontitis, 418 patients with severe periodontitis, and 260 healthy controls free of gum disease. Genomic DNA was extracted from participants' whole blood, and genotyping of TLR2/TLR4 as performed using real-time polymerase chain reaction with TaqMan MGB primer. The genotype, allele, and haplotype frequencies were compared among control, moderate periodontitis, and severe periodontitis groups. Statistical analysis was performed using chi-square and logistic regression analyses. Of the 9 polymorphic loci detected in the two genes, one, rs11536889 (G>C) in TLR4, displayed a statistically significant difference in distribution between individuals with moderate periodontitis and severe periodontitis (P<0.05). The distribution of the GG genotype in moderate periodontitis was higher than in the severe periodontitis group (P<0.05). Further, for the haplotype rs7873784, rs1927907, and rs1153688 of TLR4, the distribution of haplotype GCG was statistically different between moderate periodontitis and severe periodontitis (P<0.05, OR=1.501). These findings indicate that variation in TLR4 may affect chronic periodontitis susceptibility in a Han Chinese population.
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PMID:Toll-like receptor 4 gene polymorphism is associated with chronic periodontitis. 2613 Dec 23