Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0030305 (
pancreatitis
)
16,014
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A better understanding of the mechanisms by which colon tumor cells are able to survive exposure to drugs would be valuable for the development of new therapeutic strategies. We used differential display-PCR to compare gene expression in the drug-sensitive HT-29 colon cancer cell line and 3 drug-resistant subpopulations derived from this parental cell line. One of the genes identified is a new gene, Regenerating IV gene (
Reg IV
), and was strongly overexpressed in HT-29 drug-resistant cells. Other drug-resistant cell lines expressed
Reg IV
at a high level, whereas a low expression was noted in sensitive cell lines. Northern blot and real-time PCR analysis showed that
Reg IV
is more strongly expressed in 71% of colorectal tumors (in particular in mucinous carcinomas) than in normal colon tissues. The comparison of
Reg IV
expression with that of other REG genes, Regenerating Ialpha or (Reg Ialpha), Regenerating Ibeta (Reg Ibeta) and
Pancreatitis
-associated protein (PAP), highlights its predominant expression in colorectal tumors.
Reg IV
mRNA-positive tumor cells display different phenotypes: mucus-secreting, enterocyte-like or undifferentiated. Interestingly, whereas
Reg IV
expression is low in normal colon, its level in normal small intestine is similar to that in some colorectal tumors. In normal tissue,
Reg IV
mRNA-positive cells are mostly enteroendocrine cells and goblet cells. Our results point out the potential role of
Reg IV
in colorectal tumors and its subsequent interest as a pronostic indicator of tumor survival.
...
PMID:Reg IV, a new member of the regenerating gene family, is overexpressed in colorectal carcinomas. 1245 32
