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Query: UMLS:C0030201 (
Postoperative pain
)
1,085
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Postoperative pain
is a critical problem in clinical pain administration. Due to the unclear formation mechanism of postoperative pain, the treatment of postoperative pain is still in the symptomatic treatment stage and lacking satisfactory analgesic effect.
Postoperative pain
can be simulated by using a rat incision pain model. We observed changes in pain-related behavior of rats affected by the 5-hydroxytryptamine 2A receptor (5-HT
2A
R) agonist, TCB-2, and antagonist, ketanserin, through intrathecal delivery. The transcription and translation level of potassium-chloride cotransporter 2 (
KCC2
) in the spinal cord was also measured to investigate the role of the 5-HT
2A
R-
KCC2
pathway in the mechanism of incision pain. Compared with the control group, rats in the incision pain group had decreased mechanical withdrawal threshold (MWT), with significant differences on day 1-7 after surgery, and significant decreases in thermal withdrawal latency (TWL) on days 1, 2, 3 and 6 (P<0.05). Compared with the incision + dimethyl sulfoxide (DMSO) group, MWT and TWL decreased in the incision + ketanserin group on day 1 and 2 (P<0.05). There was no significant difference detected in TWL of incision + TCB-2 group on day 1, while MWT increased significantly compared to the incision + DMSO group (P<0.05). Furthermore, the transcription and translation levels of
KCC2
in the incision + ketanserin group decreased significantly in comparison to the incision + DMSO group (P<0.05), while an increase was detected in the incision + TCB-2 group (P<0.05). MWT and TWL decreased in the incision pain rats, accompanied with a decreased transcription and translation level of
KCC2
. Intrathecal delivery of the 5-HT
2A
R agonist, TCB-2, alleviated the decreased WMT and inhibited the decreased transcription and translation level of
KCC2
, while intrathecal delivery of the 5-HT
2A
R antagonist, ketanserin, aggravated the decreased WMT and transcription and translation levels of
KCC2
, suggesting the involement of the 5-HT
2A
R-
KCC2
pathway in the formation mechanism of incision pain in rats.
...
PMID:The 5-HT
2A
receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model. 2810 Nov 55
