Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0029713 (immaturity)
 4,335 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We applied the protein A-gold (PAG) technique to two normal human pituitary glands and three samples of hGH-producing pituitary adenoma in order to demonstrate immunoreactive hGH at the ultrastructural level. Ultrathin mirror sections immunostained for hGH were observed by electron microscopy and the numbers of immunoreactive gold particles were calculated. The numbers of gold particles of the same secretory granule shared by the mirror sections showed counts which were approximately the same. Thus, assuming that the influence on the hGH antigenicity of the tissue treatment and conditions of immunostaining should be the same at all points on the ultrathin sections, the numbers of gold particles were considered to be a quantitative indicator of the amount of hGH antigen on the secretory granules of sections processed under the same conditions. The numbers of gold particles per micron2 of cut surface of secretory granules in hGH-producing adenoma cells were statistically lower than those of hGH-positive cells from normal pituitary glands, suggesting endocrinological derangement of the tumor cells together with morphological immaturity in their secretory granules and other organelles.
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PMID:Quantitative analysis of immunoreactive human growth hormone (HGH) in the secretory granules of normal pituitary glands and hgh-producing pituitary adenomas. An immunoelectron microscopic study using the protein A-gold (PAG) technique. 283 4

The binding of human [125I]GH, ovine [125I]GH, bovine [125I]GH, and ovine [125I]PRL, to hepatic microsomal membranes (100,000 g) prepared from fetal, neonatal, and infant lambs and adult ewes has been examined. The specific binding of hGH increases (P less than 0.01) from 3.4 +/- 0.8% in the fetus (n = 7) to 20.0 +/- 2.1% (n = 6) in lambs at least 6 days postpartum. The binding of oGH is low in the fetus (0.7 +/- 0.2%; n = 13) and neonatal lamb (1.3 +/- 0.5%; n = 5) and increases (P less than 0.01) in older lambs (14.6 +/- 4.7%; n = 6) and adult sheep (14.9 +/- 5.3%; n = 4). Similarly, the binding of bGH is less (P less than 0.05) to fetal tissues. In contrast, the binding of oPRL is similar in fetal and postnatal preparations. Cross-reaction studies suggest that the binding of GHs is to a site with lactogenic characteristics in the fetus. In contrast, in lambs at least 4 days postpartum and in adult sheep, binding is to a site with somatogenic characteristics. The inability to detect somatogenic sites in the fetal liver is not due to saturation by endogenous GH or chorionic somatomammotropin, as the binding characteristics do not change after MgCl2 pretreatment of the membrane fractions. No change in binding is observed 25 days after fetal decapitation at 69 days (n = 3), suggesting that circulating GH does not down-regulate the fetal GH receptor. These observations suggest an immaturity of the somatogenic receptor in the ovine fetal liver and its appearance in the perinatal period. Immaturity of this receptor is likely to be the basis for the lack of a major effect of fetal GH on fetal somatic growth.
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PMID:The ontogeny of somatotropic binding sites in ovine hepatic membranes. 613 14