Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0029713 (immaturity)
 4,335 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The "in situ" kinetic constants (app. Km and Vmax) of brush border neutral alpha-glucosidase (EC 3.2.1.20) and lactase/beta-glucosidase (EC 3.2.1.21) were determined 4,6 (only alpha-glucosidase), and 12 days after 60% proximal intestinal resection in rat ileum at the villus base and the transition zone between middle and upper villus third by use of a quantitative biochemical analysis of enzymes in tissue sections (section biochemistry). Sham-operated rats served as controls, and the kinetic data (means per rat, time and villus position) were compared (n = 4 animals in each experimental group) first by an overall factorial analysis of variance and thereafter in detail using nonparametric test procedures. Both enzyme activities exhibited a differential response: No changes of lactase/beta-glucosidase kinetics, but a significant decrease in both Vmax- and Km-values of neutral alpha-glucosidase, which was already fully expressed on day 4 after resection and confined to the apical villus region still implying a basoapical increase of Vmax and thus maintaining the normal activity gradient on a lower level. In conclusion, a complex pattern of enzymatic adaptation to proximal intestinal resection ensues in the hyperplastic ileal mucosa which cannot be explained simply in terms of the hypothesis of cellular immaturity.
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PMID:The adaptive response of disaccharidase activities at different sites along the villus epithelium after proximal intestinal resection in the rat. A microdensitometric study of enzyme kinetics. 641 38