Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0029713 (immaturity)
 4,335 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

With the aim of improving the treatment of AML patients the activities of selected nucleoside metabolizing enzymes of possible implication for the initial metabolism of Ara-C have been studied in leukemic and normal cells. An increased ADA activity, a slightly changed PNP activity, a decreased CDD activity and an increased dCK activity, including deviation in substrate specificity, were found in myeloid leukemic cells. The increase of ADA may in part be related to immaturity of the cells. This is supported by the finding of a decrease in the ADA activity during experimental induced differentiation of myeloid cells. On the other hand differentiation is not dependent on the ADA decrease, since differentiation can occur without the decrease of ADA. The correlation between myeloid blast counts in peripheral blood from AML patients and the ADA activity also supports the connection between the development stage of the cells and the ADA activity. Minor changes of the ADA activity in myeloid cells from patients with liver cirrhosis have been observed without changes in the maturity of the cells indicating that other factors than the differentiation of the cells are of importance for the activity of ADA in myeloid cells. Only minor changes have been observed in the activity of PNP related to myeloid leukemic cells. CDD activity is decreased in myeloid leukemic cells. Differentiation is probably of major importance for the activity, experimentally supported by the increase observed concurrently with induced differentiation of myeloid cells. An inverse correlation between the CDD activity and the blast counts in patients with AML is also supportive.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Features of the initial metabolism of 1-beta-D-arabinofuranosylcytosine in human myeloid leukemic cells. 202 53

Some purine metabolizing enzymes of lymphocytes and granulocytes were determined in 13 patients with cirrhosis of the liver and in a control group consisting of 18 healthy blood donors. Furthermore cytidine deaminase (EC 3, 5, 4, 5) (CRD) activity was determined in the granulocytes of these patients and in 16 controls. An increase of adenosine deaminase (EC 3, 5, 4, 4) (ADA) activity was found in granulocytes (P less than 0.01) as well as in lymphocytes (P less than 0.01) of the cirrhotic patients as compared to controls. Purine nucleoside phosphorylase (EC 2, 4, 2, 1) (PNP) activity in granulocytes and lymphocytes was identical in the two groups. In lymphocytes of cirrhotic patients decreased hypoxanthine guanine phosphoribosyltransferase (EC 2, 4, 2, 8) (HGPRT) (P less than 0.01), adenine phosphoribosyltransferase (EC 2, 4, 2, 7) (APRT) (P less than 0.02) and adenosine kinase activities (EC 2, 7, 1, 20) (AK) (P less than 0.05) were demonstrated. 5'-nucleotidase (5'-N (EC 3, 1, 3, 5) activity in lymphocytes of cirrhotic patients was slightly increased, the increase being correlated to the level of serum gamma globulin. Granulocytes from cirrhotic patients showed a decrease of CRD (P less than 0.05). The finding that ADA activity is increased in mature lymphocytes and granulocytes from cirrhotic patients argues against the possibility that increase of lymphocytes ADA activity is a consequence of malignant transformation or immaturity.
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PMID:Changes in some nucleoside metabolizing enzymes of lymphocytes and granulocytes from patients with cirrhosis of the liver. 641 76