Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0029463 (
osteosarcoma
)
16,637
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
F-box proteins are essential components of the Skp-cullin-F-box complex (a type of E3 ubiquitin ligase), and participate in cell cycle and immune responses through the ubiquitin proteasome system.
F-box protein 39
(
FBXO39
) belongs to the F-box family, which has been reported to be associated with cancer oncogenesis and progression. The present study aimed to investigate the role of
FBXO39
in
osteosarcoma
(OS) cell proliferation and apoptosis
in vitro
. It was demonstrated that U-2OS cells exhibited high expression of
FBXO39
compared with HOS and SaOS-2
osteosarcoma
cells. Thus, knockdown of
FBXO39
was performed using lentivirus-mediated short hairpin RNA (shRNA) transfection to validate the effect of
FBXO39
in U-2OS cells. Western blotting and RT-qPCR analysis were used to confirm the efficiency of infection by analyzing the expression level of
FBXO39
. Using Celigo-based cell counting and MTT assays, it was demonstrated that
FBXO39
knockdown significantly reduced the rate of cell proliferation compared with control. Caspase 3/7 activity assays and fluorescence-activated cell sorting confirmed the induction of apoptosis in U-2OS cells following
FBXO39
knockdown. In conclusion, it was demonstrated that
FBXO39
knockdown may significantly inhibit proliferation and promote apoptosis of U-2OS cells. Thus,
FBXO39
may serve an important role in OS progression.
...
PMID:Knockdown of FBXO39 inhibits proliferation and promotes apoptosis of human osteosarcoma U-2OS cells. 3000 75
