Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0029463 (osteosarcoma)
 16,637 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

114 patients with osteosarcoma in the extremities had been reported to the SSG II trial, 132 to the SSG VIII trial and, until October 1998, 99 to the ISG/SSG I trial. The SSG IV trial included 53 patients and the SSG IX trial 104 patients until October 1998. In the SSG II trial, 19% were good responders (grades III and IV) as compared to 51% in the SSG VIII trial. On reevaluation was the response changed in one forth of the cases in both the SSG II and SSG VIII trials. In 9 and 10 cases (8%), respectively, the reevaluation resulted in a change from "good responder" to "bad responder". In the ISG/SSG I trial, the preliminary results showed a good response in 22% of the cases. In the SSG IV trial, 44% were good responders (grades III and IV), as compared to 54% in the SSG IX trial.
 ...
PMID:Diagnosis and tumor response in osteosarcoma and Ewing's sarcoma, according to treatment protocols SSG II, SSG VIII, ISG/SSG I, SSG IV and SSG IX. 1042 18

In the present multicenter study from the Scandinavian Sarcoma Group, local recurrence was analyzed regarding a change in the chemotherapy protocol and an increasing number of limb-salvage procedures 1982-97. Surgery was performed at 13 hospitals in Scandinavia. We analyzed the data of 223 patients with non-metastatic, high-grade osteosarcoma of the extremities, treated according to the SSG II and VIII protocols. The rate of limb-salvage surgery was 0.3 in SSG II, as compared to 0.6 in SSG VIII. The local recurrence rates of the limb-salvage patients were 10% (SSG II) and 11% (SSG VIII), as compared to 4% and 2%, respectively, among the amputated patients. We found no significant difference in outcome i.e., in local recurrence and survival rate despite an increased rate of good responders in SSG VIII, as compared to SSG II. It may be shown that the continuously increasing use of limb-salvage surgery is associated with a higher rate of local recurrence than with ablative surgery, despite better chemotherapy.
 ...
PMID:Surgical procedure and local recurrence in 223 patients treated 1982-1997 according to two osteosarcoma chemotherapy protocols. The Scandinavian Sarcoma Group experience. 1042 24

Distribution of mitochondria as well as other intracellular organelles in mammalian cells is regulated by interphase microtubules. Here, we demonstrate a role of microtubules in the mitochondrial biogenesis using various microtubule-active drugs and human osteosarcoma cell line 143B cells and rat liver-derived RL-34 cells. Depolymerization of microtubules by nocodazole or colchicine, as well as 2-methoxyestradiol, a natural estrogen metabolite, arrested asynchronously cultured cells in G(2)/M phase of cell cycle and at the same time inhibited the mitochondrial mass increase and mtDNA replication. These drugs also inhibited the mitochondrial mass increase in the cells that were synchronized in cell cycle, which should occur during G(1) to G(2) phase progression in normal conditions. However, stabilization of microtubules by taxol did not affect the proliferation of mitochondria during the cell cycle, yet a prolonged incubation of cells with taxol induced an abnormal accumulation of mitochondria in cells arrested in G(2)/M phase of cell cycle. Taxol-induced accumulation of mitochondria was not only demonstrated by mitochondria-specific fluorescent dyes but also evidenced by the examination of cells transfected with yellow fluorescent protein fused with mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (pEYFP) and by enhanced mtDNA replication. Two subpopulations of mitochondria were detected in taxol-treated cells: mitochondria with high Delta(psi)(m), detectable either by Mito Tracker Red CMXRos or by Green FM, and those with low Delta(psi)(m), detectable only by Green FM. However, taxol-induced increases in the mitochondrial mass and in the level of acetylated (alpha)-tubulin were abrogated by a co-treatment with taxol and nocodazole or taxol and colchicine. These data strongly suggest that interphase microtubules may be essential for the regulation of mitochondrial biogenesis in mammalian cells.
 ...
PMID:Opposite effects of microtubule-stabilizing and microtubule-destabilizing drugs on biogenesis of mitochondria in mammalian cells. 1114 30

Bone matrix proteins are being identified in mineralized foci in several different pathological states. Irrespective of how these proteins become localized it is of great interest to further define what endocrine factors are necessary for triggering the release of bone matrix proteins. MG63 cells (Osteosarcoma cell line) were seeded at a density of 1 x 10(5) cells/ml onto two 24 well tissue culture plates. The wells were divided into 8 groups of 6 wells/group. Cells in group 1 were provided with media alone and served as a control. Cells in group II, IV, and VI were supplemented with Insulin Like Growth factor-1 (IGF-1), Testosterone (T), or Growth Hormone (GH), respectively. Cells in groups III, V and VII were supplemented with TCPL capsules containing IGF-1, Test or GH, respectively. Cells in group VIII were incubated with sham TCPL and served as vehicle control. Cells were incubated in the presence or absence of hormones for 24, 48 and 72 hours. At the end of each phase, cell damage, cell morphology, cell number and cellular protein concentrations were determined. The results showed a significant increase in MDA initially in all groups, followed by a drop in MDA levels by the 48-hour phase, and thereafter remaining lower than the control. Results also demonstrated a decrease in total cellular protein following administration of hormones by conventional means. Sustained delivery of the hormones by TCPL resulted in only a slight increase in protein levels. Cell count data showed an initial decrease in all groups, followed by a significant increase in cell number at the end of 48 hours and, finally, reaching control levels at the 72 hour period. Morphological evaluation revealed major structural changes associated with sustained delivery as compared to conventional delivery of hormones. For example, sustained delivery of IGF-1 and T resulted in an increase in mitotic figures, swelling, and aggregate formation. Cluster cellular formation were observed on cells exposed to IGF-1 and T by conventional routes.
 ...
PMID:The effects of sustained delivery of growth promoting hormones on the proliferation of MG63 cells in culture. 1134 1

Data on 5572 children and adolescents diagnosed with malignant bone tumours (International Classification of Childhood Cancer, Group VIII) before the age of 20 years during 1978-1997 in Europe were extracted from the Automated Childhood Cancer Information System (ACCIS) database. Age-standardised incidence among children during the period 1988-1997 was similar for boys and girls aged 0-14 years (5.5-5.6 per million). Among adolescents aged 15-19 years, males had higher incidence (19.3 per million) than females (10.7 per million). Among children, osteosarcoma accounted for 51% of registrations and Ewing's sarcoma for 41%. Among adolescents, 55% of registrations were osteosarcoma and 28% Ewing's sarcoma. Both tumours had their highest incidence in late childhood or early adolescence. There were no significant time trends in incidence during 1978-1997. Five-year survival estimates for patients diagnosed during 1988-1997 were, respectively, 59% and 51% among children and adolescents with osteosarcoma and 62% and 30% among children and adolescents with Ewing's sarcoma. Between 1978-1982 and 1993-1997, survival increased for both children and adolescents with osteosarcoma, and for children with Ewing's sarcoma.
 ...
PMID:Bone tumours in European children and adolescents, 1978-1997. Report from the Automated Childhood Cancer Information System project. 1691 76

A simple and rapid method using high-speed counter-current chromatography (HSCCC), along with bioassay-guided fractionation based on the anti-proliferative activity against renal and colon cancer cells, has been developed for the preparative separation of aceroside VIII (1) and platyphylloside (2) from Betula platyphylla. A solvent system composed of ethyl acetate/acetonitrile/water (1:0.1:1, v/v/v) was optimized for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. Among these isolated diarylheptanoids, platyphylloside (2) showed anti-proliferative activity in the COLO205 and KM12 colon cells and renal cancer cell lines A498, U031, as well as in MG63 and MG 63.3 osteosarcoma cells. In addition, it showed dose dependent inhibitory effects in the NCI 60 cell line assay. These results suggest that the diarylheptanoids isolated from B. platyphylla with an efficient HSCCC method could be potential multi-targeted therapeutic agents for cancer.
...
PMID:Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography. 2724 Mar 38