Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0029463 (
osteosarcoma
)
16,637
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Osteosarcoma
is the most common type of primary malignant bone tumor and has a high propensity to metastasize to the lungs and bones. Calbindin 1 (
CALB1
) is a constituent Ca2+ binding protein, which can prevent apoptotic death in several cell types induced through various pro-apoptotic signaling pathways. To investigate whether
CALB1
is implicated in the tumor growth of human
osteosarcoma
, two different short hairpin RNAs (shRNAs) against
CALB1
were used for
CALB1
-knockdown in
osteosarcoma
U2OS cells. The U2OS cells were divided into three groups: Two groups with
CALB1
knockdown (
CALB1
-shRNA 1 and
CALB1
-shRNA 2) and one control group (Con-shRNA). Reverse transcription-quantitative polymerase chain reaction and western blot analysis confirmed that the
CALB1
-shRNA 1- and 2-infected cells exhibited significantly lower levels of
CALB1
gene and protein expression compared with the Con-shRNA group. The proliferation and colony formation abilities were significantly inhibited in
CALB1
-deficient U2OS cells compared with the control, as measured using an MTT assay and crystal violet staining. Flow cytometry revealed that the number of
CALB1
-shRNA 2-injected cells was increased in the G
0
/G
1
and G
2
/M phases, but decreased in the S phase, compared with the control group. The assessment of apoptosis and necrosis using Annexin V/7-aminoactinomycin D demonstrated that there was a significantly higher percentage of necrotic, early apoptotic, and late apoptotic cells, but a significantly lower percentage of viable cells in U2OS cells with
CALB1
-knockdown compared with the control group. In conclusion,
CALB1
contributes to protecting
osteosarcoma
cells from apoptosis and provides a potential novel target for gene therapy to treat patients with
osteosarcoma
.
...
PMID:Downregulation of calbindin 1, a calcium-binding protein, reduces the proliferation of osteosarcoma cells. 2852 88
