Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0029463 (osteosarcoma)
 16,637 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This work focused on studying the effect of blending gelatin (Gel) with Cellulose (Cel), in the presence of montmorillonite (MMT), on the swelling behavior, in vitro degradation and surface morphology. Additionally, the effect of the prepared biocomposites on the characteristics of the human osteosarcoma cells (Saos-2), including proliferation, scaffold/cells interactions, apoptosis and their potential of the cells to induce osteogenesis and differentiation was evaluated. The crosslinked biocomposites with glutaraldehyde (GA) or N,N-methylene-bisacrylamide (MBA) was prepared via an intercalation process and freeze-drying technique. Properties including SEM morphology, X-ray diffraction characterization and in vitro biodegradation were investigated. The successful generation of 3-D biomimetic porous scaffolds incorporating Saos-2 cells indicated their potential for de novo bone formation that exploits cell-matrix interactions. In vitro studies revealed that the scaffolds containing 12 and 6% MMT crosslinked by 5 and 0.5% GA seem to be the two most efficient and effective biodegradable scaffolds, which promoted Saos-2 cells proliferation, migration, expansion, adhesion, penetration, spreading, and differentiation, respectively. MMT improved cytocompatibility between the osteoblasts and the biocomposite. In vitro analysis indicated good biocompatibility of the scaffold and presents the scaffold as a new potential candidate as suitable biohybrid material for tissue engineering.
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PMID:Preparation, characterization and in vitro biological study of biomimetic three-dimensional gelatin-montmorillonite/cellulose scaffold for tissue engineering. 1962 50

In recent years, plant based scaffold due to its inherent properties such as mechanical stability, renewability, easy mass production, inexpensiveness, biocompatibility and biodegradability with low toxic effects have received much attention in the field of bone tissue engineering. Design of good tissue compatible plant based polymer scaffold plays a vital role in biomedicine, nanomedicine and in various tissue engineering applications. The present study focused on the fabrication of a novel herbal scaffold using the medicinal plants Spinacia oleracea (SO) and Cissus quadrangularis (CQ) extracts incorporated with Alginate (Alg), Carboxy Methyl Cellulose (CMC) by lyophilization method. The structural nature and the properties of prepared scaffold were analyzed by XRD, FE-SEM, FTIR, EDAX, TGA, swelling ratio, porosity, in-vitro degradation and cell viability studies. The biocompatible nature of the plant based polymer scaffold was assessed using MG-63 Human Osteosarcoma cell line. The investigation of biocompatibility study showed that Alg/CMC/SO scaffold expressed higher cell viability than Alg/CMC/SO-CQ scaffold, which possess better cellular biocompatibility. The results of the present study suggested that plant based Alg/CMC/SO scaffold serve as a potential biopolymer scaffold which could be further exploited for bone tissue applications.
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PMID:Fabrication and characterization of Spinacia oleracea extract incorporated alginate/carboxymethyl cellulose microporous scaffold for bone tissue engineering. 3229 96