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Query: UMLS:C0029463 (osteosarcoma)
 16,637 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Silver (or platinum)-containing calcium phosphate (hydroxyapatite (HA) and tricalcium phosphate (alpha-TCP)) coatings on titanium substrates were formed by micro-arc oxidation (MAO) and their in vitro antibacterial activity and in vitro cytotoxicity were evaluated. MAO was performed in an electrolytic solution containing beta-glycerophosphate disodium salt pentahydrate (beta-GP) and calcium acetate monohydrate (CA), and Ag and Pt were introduced in the form of AgNO(3) (or CH(3)COOAg) and H(2)PtCl(6), respectively. The MG63 and human osteosarcoma (HOS) cell lines were used to investigate the proliferation and differentiation behavior of the cells, respectively, whereas two strains of bacteria, Staphylococcus aureus and Escherichia coli, were used to evaluate the antibacterial activity of the coatings. The phase, morphology, and Ag content of the coatings were strongly dependent on the applied voltage and Ag precursor concentration. HA and alpha-TCP phases were detected in the coatings oxidized above 400 V and the presence of Ag was confirmed by EDS. While the coatings with a high content of Ag were cytotoxic and those obtained in the Pt-containing electrolyte had no apparent antibacterial activity, the calcium phosphate coatings obtained in the low Ag concentration electrolyte exhibited in vitro antibacterial activity but no cytotoxicity. Thus, biocompatible calcium phosphate coatings on Ti implants with antibacterial activity can be achieved by one-step MAO.
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PMID:Antibacterial properties of Ag (or Pt)-containing calcium phosphate coatings formed by micro-arc oxidation. 1828 18

Anisotropic cell culture surfaces patterned with amino and alkylsilanes can guide cell distribution and provide an approach to study important processes involved in tissue engineering, such as cell attachment and locomotion. By combining photolithographic and silane coupling techniques, glass coverslips were patterned with either n-octadecyldimethylchlorosilane (ODDMS) or dimethyldichlorosilane (DMS), and N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane (EDS). The alkylsilanes, theoretically, have similar methyl and methylene groups exposed at the surface but different structures, with DMS being amorphous and ODDMS ordered. Neuroblastoma cells, osteosarcoma cells, and fibroblasts plated on surfaces patterned with EDS/ODDMS and EDS/DMS specifically localized on the EDS regions, but distributed randomly on ODDMS/DMS patterned surfaces. The preferential assembly of cells onto EDS regions did not depend on the structure of the adjacent alkylsilane regions and was a time-dependent process. Angle dependent x-ray photoelectron spectroscopy (XPS) and contact angle measurements indicated that EDS was immobilized on glass as a fractional hydrophilic monolayer, and ODDMS and DMS were bound as patchy amorphous hydrophobic multilayers. Neither surface coverage nor thickness of the overlayer seemed to be as important as surface chemistry, or charge, in guiding mammalian cell distribution. These results are consistent with the concept that mammalian cells attach to and are guided by positively charged surfaces.
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PMID:Spatial distribution of mammalian cells dictated by material surface chemistry. 1861 3

Chitosan, a deacetylated derivative of chitin is a commonly studied biomaterial for tissue-engineering applications due to its biocompatibility, biodegradability, low toxicity, antibacterial activity, wound healing ability and haemostatic properties. However, chitosan has poor mechanical strength due to which its applications in orthopedics are limited. Hydroxyapatite (HAp) is a natural inorganic component of bone and teeth and has mechanical strength and osteoconductive property. In this work, HAp was deposited on the surface of chitosan hydrogel membranes by a wet chemical synthesis method by alternatively soaking the membranes in CaCl(2) (pH 7.4) and Na(2)HPO(4) solutions for different time intervals. These chitosan hydrogel-HAp membranes were characterized using SEM, AFM, EDS, FT-IR and XRD analyses. MTT assay was done to evaluate the biocompatibility of these membranes using MG-63 osteosarcoma cells. The biocompatibility studies suggest that chitosan hydrogel-HAp composite membranes can be useful for tissue-engineering applications.
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PMID:Wet chemical synthesis of chitosan hydrogel-hydroxyapatite composite membranes for tissue engineering applications. 1944 53

A novel biomimetic f-HAp/PVP/Ag nanocomposite was synthesized under the ionic liquid medium, which was composed of inorganic and organic nanofillers like fluor-hydroxyapatite, silver nanoparticles and polyvinyl pyrrolidone. In composite synthesis, the first time we were used fluorine based ionic liquid for the fluorine contents on the fluor-hydroxyapatite nanoparticles which were resulting in very good crosslinking and interfacial bonding with the PVP and Ag nanoparticles. Ionic liquid has assisted good morphological structure of both inorganic materials. The chemical interaction and crystallinity changes of the nanocomposite were evaluated by FTIR and XRD studies. The surface morphology and composition of the samples were observed by FE-SEM, HR-TEM and EDS analyses. This report reveals that the greener approach for synthesis of fluor-hydroxyapatite nanocomposite and sustained delivery of silver and fluorine ions from the fluor-hydroxyapatite surface to the bacterial surface have been reducing the bacterial growth rate which was evaluated by different pathogenic bacterial strains via different methods and it also favourable cytotoxicity effect with human osteosarcoma (MG-63) cells.
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PMID:Novel strategy for f-HAp/PVP/Ag nanocomposite synthesis from fluoro based ionic liquid assistance: Systematic investigations on its antibacterial and cytotoxicity behaviors. 2728 93

Fused deposition modeling (FDM) is a promising 3D printing and manufacturing step to create well interconnected porous scaffold designs from the computer-aided design (CAD) models for the next generation of bone scaffolds. The purpose of this study was to fabricate and evaluate a new biphasic calcium phosphate (BCP) scaffold reinforced with zirconia (ZrO2 ) by a FDM system for bone tissue engineering. The 3D slurry foams with blending agents were successfully fabricated by a FDM system. Blending materials were then removed after the sintering process at high temperature to obtain a targeted BCP/ZrO2 scaffold with the desired pore characteristics, porosity, and dimension. Morphology of the sintered scaffold was investigated with SEM/EDS mapping. A cell proliferation test was carried out and evaluated with osteosarcoma MG-63 cells. Mechanical testing and cell proliferation evaluation demonstrated that 90% BCP and 10% ZrO2 scaffold had a significant effect on the mechanical properties maintaining a structure compared that of only 100% BCP with no ZrO2 . Additionally, differentiation studies of human mesenchymal stem cells (hMSCs) on BCP/ZrO2 scaffolds in static and dynamic culture conditions showed increased expression of bone morphogenic protein-2 (BMP-2) when cultured on BCP/ZrO2 scaffolds under dynamic conditions compared to on BCP control scaffolds. The manufacturing of BCP/ZrO2 scaffolds through this innovative technique of a FDM may provide applications for various types of tissue regeneration, including bone and cartilage.
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PMID:Fabrication and evaluation of 3D printed BCP scaffolds reinforced with ZrO2 for bone tissue applications. 2924 Feb 43

This report has approached for the green synthesis of morphological controlled novel metal-doped fluorinated apatite/polymeric nanocomposites. The synthesized nanocomposites have investigated for hard tissue engineering and bone substitute applications. The selected fluoro ionic liquid explored the dual performances as fluorine precursor and as a soft template for the morphological development of apatite nanocomposite synthesis. The structural and surface studies (XRD, FTIR, FE-SEM, EDS, AFM, HR-TEM & SAED) confirmed the crystalline and morphological changes of synthesized fluorohydroxyapatite nanostructures at two different reaction temperatures. The fluorinated apatite nanocomposites doped with silver for metal-doped composites, which have effective antibacterial efficacy and favorable biocompatibility. The silver-doped nanocomposites showed excellent antibacterial ability against Staphylococcus aureus and Escherichia coli bacterial pathogens with the uniform release of silver and fluorine ions. These antibacterial performances have systematically tested by the quantitative and qualitative methods. The rod-like fluorinated apatite nanocrystals promote cell adhesion and viability of human osteosarcoma (MG-63) cell lines and these studies compared with the sheet-like apatite nanocomposites. This type of biomedical apatite materials may be a promising material for orthopedic implant and regeneration applications.
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PMID:Influences of ionic liquid and temperature on the tailorable surface morphology of F-apatite nanocomposites for enhancing biological abilities for orthopedic implantation. 2951 48