Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0029463 (
osteosarcoma
)
16,637
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Objective:
Osteosarcoma
is one of the most common malignancies in children and adolescents. Studies have shown that miR-34c-5p is involved in the progression of various cancers. To explore the effects of miR-34c-5p on the proliferation, migration and invasion of
osteosarcoma
cells and its potential mechanism.
Methods:
qRT-PCR was used to detect the expression levels of miR-34c-5p and
FLOT2
mRNA in
osteosarcoma
tissues and cells. Western Blot was used to detect protein expression. MTT assay used to detect cell viability. Transwell was used to detect cell migration and invasion in each group. Dual luciferase reporter gene assay was used to detect luciferase activity.
Results:
The expression of miR-34c-5pwas significantly decreased in
osteosarcoma
tissues and cells and the expression level of
FLOT2
mRNA was significantly increased. Overexpression of miR-34c-5p and inhibition of
FLOT2
inhibited the proliferation, migration and invasion of
osteosarcoma
cells and inhibited the expression of Cyclin D1, MMP-2 and MMP-9 proteins and promoted the expression of p21 protein. miR-34c-5p targeted to regulate the expression of
FLOT2
. Overexpression of
FLOT2
reversed the inhibitory effect of miR-34c-5p overexpression on proliferation, migration and invasion of
osteosarcoma
cell lines.
Conclusion:
miR-34c-5p can inhibit the proliferation, migration and invasion of
osteosarcoma
cells. The mechanism may be related to targeting
FLOT2
, which will provide a new target for the prevention and treatment of
osteosarcoma
.
...
PMID:The study of mechanism of miR-34c-5p targeting FLOT2 to regulate proliferation, migration and invasion of osteosarcoma cells. 3144 95
