Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0029463 (osteosarcoma)
 16,637 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Pentavalent 99Tcm-dimercaptosuccinic acid (99Tcm-(V)DMSA) has established uses in the detection and diagnosis of medullary thyroid carcinoma, osteosarcoma, amyloidosis and many soft tissue tumours, but is not readily available as a commercial kit. We have evaluated published methods for preparation of 99Tcm-(V)DMSA that are based on the modification of 99Tcm-(III)DMSA commercial kits. The criteria for assessment were achievement of satisfactory radiochemical purity and practical considerations regarding the ease of synthesis. Quantification of the desired product's activity was achieved via thin-layer chromatography (silica gel) with an n-butanol:acetic acid:water solvent system. The developed plates were imaged on a gamma camera and analysed using standard software. The methods investigated were designated as Birmingham, Cambridge and Canterbury: the latter two were very similar. With experience, the methods eventually produced similar radiochemical purities of > 90%. However, the Birmingham method was found to be more satisfactory in terms of its reliability and simplicity of synthesis.
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PMID:A critical evaluation of methods for preparing pentavalent 99Tcm-DMSA. 1045 86

Although the liver is the primary site of cytokine-mediated expression of acute-phase serum amyloid A (SAA) protein, extrahepatic production has also been reported. Besides its role in amyloidosis and lipid homeostasis during the acute-phase, SAA has recently been assumed to contribute to bone and cartilage destruction. However, expression of SAA in human osteogenic tissue has not been studied. Therefore, we first show that SAA1 (coding for the major SAA isoform) but not SAA2 transcripts are expressed in human trabecular and cortical bone fractions and bone marrow. Next, we show expression of (i) IL-1, IL-6, and TNF receptor transcripts; (ii) the human homolog of SAA-activating factor-1 (SAF-1, a transcription factor involved in cytokine-mediated induction of SAA genes); and (iii) SAA1/2 transcripts in non-differentiated and, to a higher extent, in osteoblast-like differentiated human mesenchymal stem cells. Third, we provide evidence that human osteoblast-like cells of tumor origin (MG-63 and SAOS-2) express SAF-1 under basal conditions. SAA1/2 transcripts are expressed under basal conditions (SAOS-2) and cytokine-mediated conditions (MG-63 and SAOS-2). RT-PCR, Western blot analysis, and immunofluorescence technique confirmed cytokine-mediated expression of SAA on RNA and protein level in osteosarcoma cell lines while SAA4, a protein of unknown function, is constitutively expressed in all osteogenic tissues investigated.
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PMID:Expression of serum amyloid A transcripts in human bone tissues, differentiated osteoblast-like stem cells and human osteosarcoma cell lines. 1784 29