Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0027960 (mole)
 21,279 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The carbohydrate of variant-specific surface antigen glycoproteins from bloodstream forms of 13 cloned variants of Trypanosoma brucei was analyzed by gas-liquid chromatography. The glycoproteins contained from 6 to 17% carbohydrate by weight, and all contained the same 4 sugars: mannose, galactose, glucose, and glucosamine (probably as N-acetylglucosamine). The glycoprotein from variant 048, strain 427 contained (+20%) 11 mannose, 4 galactose, 4 glucose, and 5 glucosamine residues/mole of glycoprotein (molecular weight 65,000). Glucose was an intergral component of the glycoproteins, not dissociable by sodium dodecyl sulphate, 8 M urea, or 1 M acetic acid. Some of the glucose was dissociated by trichloroacetic acid. Most of the glycoproteins formed precipitin with concanavalin A in Ouchterlony double diffusion, but none formed such bands with wheat germ agglutinin or Ricinus communis lectin (molecular weight 120, 000).
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PMID:Carbohydrate composition of variant-specific surface antigen glycoproteins from Trypanosoma brucei. 59 4

D 47 is a monoclonal antibody (IgG2) reacting with a surface antigen of cortical thymocytes. On normal human skin, D 47 was found to react with a cytoplasmic antigen of the cells of the secretory portion of eccrine sweat glands (ESG). No reactivity with D 47 is detected on the excretory part of ESG, on apocrine glands and all other cutaneous structures. In this work we studied through an indirect immunofluorescence method on frozen skin sections the reactivity pattern of D 47 on a group of epithelial skin tumours of certain or alleged glandular differentiation. These consisted of: eccrine spiradenoma (ES, 1 case); chondroid syringoma (CS, 1 case); syringomas of the eyelid (1 case); clear-cell hidradenocarcinoma (1 case); eccrine porocarcinoma (1 case); naevus sebaceus of Jadassohn (2 cases, one of which associated to a syringocystadenoma papilliferum); clear-cell acanthoma (1 case); extramammary Paget's disease (3 cases); basal-cell epithelioma (2 cases). D 47 yielded in a strong labelling of a significant proportion of cells in the cases of ES and CS, while on the remaining tumours, apart from normal ESG occasionally present in the peritumoral connective tissue, no reactivity was seen. From this study it becomes clear that D 47 represents an immunohistologic marker of eccrine-secretory differentiation and that it can be applied in the investigation of the differentiation and of the differential diagnosis of sweat-gland neoplasms.
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PMID:[Contribution of monoclonal antibody D 47 in the study of sweat gland pathology]. 299 Apr 99

The aim of this work is to identify and purify cultured nevus cells. We used for our study monoclonal antibody 225.28S. This antibody reacts with a surface antigen which is expressed by nevus cells and melanoma cells, but it does not react with normal melanocytes. We studied 10 dermic nevi and we observed that antigenic determinant survives in cultured nevus cells. These results allowed us to employ the method of panning for purify cultured nevus cells.
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PMID:[Use of monoclonal antibody 225.28S in the study of nevus cells in culture]. 637 Feb 74