Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0027960 (mole)
 21,279 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Thiaminase (EC 2.5.1.2) from freshwater bivalve molluscs is a polycosubstrate enzyme, which is resistant to temperatures below 55 degrees. The activation energy of the enzyme with cystein is 15077 cal/mole, with aniline--15948 cal/mole. Thiaminase when injected parenterally to albino mice 2 hrs after injection of [2-14C]thiamine causes an extensive destruction of labelled vitamin b1, which is evidenced from the [14C]thiazole content in urine, liver and kidneys. The total radioactivity and the content of [14C]thiazole in the urine samples collected within 6 hrs after thiamine injection are increased. Cysteine and histidine in combination with thiaminase have no activating effect similar to that observed "in vitro". In the experimental series when non-labelled vitamin B1 from animal tissues (liver, kidney, spleen, muscle) was substituted by the labelled one, the parenterally injected enzyme destroyed up to 30-50% of total thiamine content. Brain and skeletal muscle tissues are more resistant to thiaminase action.
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PMID:[Direct measurement of the antivitamin action of parenterally injected thiaminase]. 724 48