Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0027960 (
mole
)
21,279
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A new method for the determination of
guanase
is described. Xanthine, the product of the
guanase
reaction, is oxidized by xanthine oxidase, forming uric acid and hydrogen peroxide. Hydrogen peroxide is further reduced to water by catalase in the presence of ethanol. The acetaldehyde formed in this reaction step is dehydrogenated NAD or NADP dependent by aldehyde dehydrogenase. The NADH or NADPH production is measured and utilized for the calculation of the
guanase
activity. The sensitivity of the method can be doubled by the addition of uricase, which oxidizes uric acid to permit the formation of another
mole
of hydrogen peroxide.
...
PMID:A new spectrophotometric assay for enzymes of purine metabolism. II. Determination of guanase activity. 48 57
