Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0027960 (
mole
)
21,279
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Zn2+ binding to canine cardiac calsequestrin was investigated using the Zn2+ specific fluorescence dye salicylcarbohydrazone (SACH), 65Zn2+ overlay and Zn(2+)-
IDA
chromatography. Cardiac calsequestrin binds approximately 200 moles of Zn2+/
mole
of protein with the Kd = 300 microM. Zn2+ binding to calsequestrin was further confirmed by 65Zn2+ overlay and Zn(2+)-dependent aggregation of the protein. However, calsequestrin did not bind to a Zn(2+)-
IDA
-agarose column, indicating that histidine residues may not be involved in Zn2+ binding to the protein. Circular dichroism revealed only minor Zn(2+)-dependent conformational changes in calsequestrin. We conclude that calsequestrin is a Ca(2+)- and Zn(2+)-binding protein and that Zn2+ may modulate the structure and function of the protein.
...
PMID:Zn2+ binding to cardiac calsequestrin. 772 52
Ethylenediaminetetraacetic acid (EDTA) and nitrilotriacetic acid (NTA) are widely used anthropogenic chelating agents for control of metal speciation and are ubiquitous in natural waters and wastewaters. This is the first report of systematic measurement of the growth yields of a mixed culture (BNC1-BNC2) on EDTA and its biodegradation intermediates, and of Aminobacter aminovorans (aka Chelatobacter heintzii) ATCC 29600 on NTA and its biodegradation intermediates. The yields measured for BNC1-BNC2 co-culture were 75.0 g of cell dry weight (CDW) (
mole
of EDTA)(-1), 68.6 g of CDW (
mole
of ED3 A)(-1), 51.2 g of CDW (
mole
of N,N'-EDDA)(-1), 34.5 g of CDW (
mole
of ED)(-1), 26.3 g of CDW (
mole
of
IDA
)(-1), 12.2 g of CDW (
mole
of glycine)(-1), and 9.7 g of CDW (
mole
of glyoxylate)(-1). The yields measured for A. aminovorans were 44.3 g of CDW (
mole
of NTA)(-1), 37.9 g of CDW (
mole
of
IDA
)(-1), 15.2 g of CDW (
mole
of glycine)(-1), and 10.4 g of CDW (
mole
of glyoxylate)(-1). The biodegradation pathways of EDTA, NTA, and several of their metabolic intermediates include reactions catalyzed by oxygenase enzymes, which may reduce energy available for cell synthesis. Comparison of measured yields with predicted yields indicates that the effect of oxygenase reaction on cell yield can be quantified experimentally as well as modeled based on thermodynamics.
...
PMID:Bacterial growth yields on EDTA, NTA, and their biodegradation intermediates. 1740 95
The stoichiometry of the oxidation of
IDA
or NTA with lead dioxide suspension was studied by polarographic measurement and by derivative polarographic titration. One
mole
and two moles of Pb(IV) are reduced per
mole
of
IDA
and NTA respectively, with moderate speed at room temperature in nitric acid solutions. One
mole
each of carbon dioxide, formaldehyde and glycine are produced from the oxidation of 1
mole
of
IDA
, and two moles of carbon dioxide, two moles of formaldehyde and one
mole
of glycine from 1
mole
of NTA. The overall reaction in each case may be written as follows: Pb(IV) +
IDA
+ H(2)O --> Pb(II) + CO(2) + HCHO + H(2)NCH(2)COOH + 2H(+) 2Pb(IV) + NTA + 2H(2)O --> 2Pb(II) + 2CO(2) + 2HCHO + H(2)NCH(2)COOH + 4H(+).
...
PMID:Studies of the oxidation of iminodiacetic acid (IDA) and nitrilotriacetic acid (NTA) with lead dioxide suspension in nitric acid. 1896 2
