Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0027819 (
neuroblastoma
)
27,800
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Sulfotransferase 4A1
(
SULT4A1
), a member of cytosolic sulfotransferases (SULT), is exclusively expressed in neurons with no known function. Severe phenotype and early postnatal death in
SULT4A1
knockout mice revealed that
SULT4A1
is an essential neuronal protein. Localization of
SULT4A1
in different cytosolic compartments, including mitochondria, suggests multiple roles for this protein. We observed that knockdown of
SULT4A1
results in the accumulation of reactive oxygen species in primary cortical neurons, suggesting a potential role of
SULT4A1
in regulating redox homeostasis. Expression of
SULT4A1
in the human
neuroblastoma
SH-SY5Y cells revealed a defused but nonuniform staining pattern in the cytoplasm, with increased density around mitochondria. Subcellular fractionation of
SULT4A1
expressing SH-SY5Y cells confirms the presence of
SULT4A1
in mitochondrial fractions.
SULT4A1
expressing cells display significant protection against H
2
O
2
-mediated defects in mitochondrial function and loss of mitochondrial membrane potential. Expression of
SULT4A1
in SH-SY5Y cells also protects against H
2
O
2
-induced cell death. These data indicate that
SULT4A1
protects mitochondria against oxidative damage and may serve as a potential pharmacological target in neural diseases involving mitochondrial dysfunction and oxidative stress. SIGNIFICANCE STATEMENT: Studies on
SULT4A1
knockout mice suggest that
SULT4A1
plays a vital role in neuronal function and survival via yet undefined mechanisms. Our data demonstrate that depletion of
SULT4A1
induces oxidative stress in neurons and expression of
SULT4A1
in SH-SY5Y cells protects against oxidative-stress-induced mitochondrial dysfunction and cell death. These results suggest that
SULT4A1
may have a crucial protective function against mitochondrial dysfunction and oxidative stress, and may serve a potential therapeutic target in different neurological diseases involving mitochondrial dysfunction and oxidative stress.
...
PMID:SULT4A1 Protects Against Oxidative-Stress Induced Mitochondrial Dysfunction in Neuronal Cells. 3126 51
