Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0027819 (
neuroblastoma
)
27,800
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
By using the yeast two-hybrid system, the zinc finger protein
ZPR9
was identified as one of the B-MYB interacting proteins that associates with the carboxyl-terminal conserved region of B-MYB.
ZPR9
was found to form in vivo complexes with B-MYB, as demonstrated by in vivo binding assay and coimmunoprecipitation experiments of the endogenously and exogenously expressed proteins. Deletion analysis revealed that this binding was mediated by all three functional domains, an amino-terminal DNA-binding domain, a transactivation domain, and a carboxyl-terminal conserved region of B-MYB. We show that the interaction of
ZPR9
with B-MYB is functional because cotransfection of
ZPR9
significantly up-regulates B-MYB transcriptional activity in a dose-dependent manner. In addition, coexpression of
ZPR9
with B-MYB caused the accumulation of B-MYB, as well as
ZPR9
, in the nucleus. Furthermore, constitutive expression of
ZPR9
in human
neuroblastoma
cells induces apoptosis in the presence of retinoic acid. These results strongly suggest that
ZPR9
plays an important role in modulation of the transactivation by B-MYB and cellular growth of
neuroblastoma
cells.
...
PMID:Enhancement of B-MYB transcriptional activity by ZPR9, a novel zinc finger protein. 1264 66
