Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0027819 (
neuroblastoma
)
27,800
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Acetoacetyl-CoA synthetase
(
AACS
) is the enzyme responsible for cholesterol and fatty acid synthesis in the cytosol. We have previously shown that
AACS
has an important role in normal neuronal development and that knockdown of SREBP-2, which orchestrates cholesterol synthesis, resulted in the downregulation of
AACS
mRNA levels. In this study, we investigated the transcriptional mechanism of
AACS
in Neuro-2a,
neuroblastoma
cells. Luciferase assay showed that the minimal core promoter of the mouse
AACS
gene is located in a region with 110 bps upstream from the transcription start site. Mutagenesis studies showed that the Sp1 binding site was crucial for
AACS
promoter activity. ChIP assay and DNA affinity precipitation assay showed that Sp1 binds to the Sp1 binding site on the promoter region of
AACS
. Moreover, overexpression of Sp1 increased
AACS
mRNA levels. Knockdown of
AACS
resulted in a decrease in histone deacetylase 9, associated with gene silencing. These results suggest that Sp1 regulates gene expression of
AACS
in Neuro-2a cells and ketone body utilization affects the balance of histone acetylation.
...
PMID:Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells. 2913 83
