Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0027819 (
neuroblastoma
)
27,800
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The expression and biological functions of oncofetal markers GD2 and GD3 were extensively studied in neuroectoderm-derived cancers in order to characterize their potential as therapeutic targets. Using immunological approaches, we previously identified GD3, GD2, and
O
AcGD2 expression in breast cancer (BC) cell lines. However, antibodies specific for
O
-acetylated gangliosides are not exempt of limitations, as they only provide information on the expression of a limited set of
O
-acetylated ganglioside species. Consequently, the aim of the present study was to use structural approaches in order to apprehend ganglioside diversity in melanoma,
neuroblastoma
, and breast cancer cells, focusing on
O
-acetylated species that are usually lost under alkaline conditions and require specific analytical procedures. We used purification and extraction methods that preserve the
O
-acetyl modification for the analysis of native gangliosides by MALDI-TOF. We identified the expression of GM1, GM2,
GM3
, GD2, GD3, GT2, and GT3 in SK-Mel28 (melanoma), LAN-1 (
neuroblastoma
), Hs 578T, SUM 159PT, MDA-MB-231, MCF-7 (BC), and BC cell lines over-expressing GD3 synthase. Among
O
-acetylated gangliosides, we characterized the expression of
O
AcGM1,
O
AcGD3,
O
AcGD2,
O
AcGT2, and
O
AcGT3. Furthermore, the experimental procedure allowed us to clearly identify the position of the sialic acid residue that carries the
O
-acetyl group on b- and c-series gangliosides by MS/MS fragmentation. These results show that ganglioside
O
-acetylation occurs on both inner and terminal sialic acid residue in a cell type-dependent manner, suggesting different
O
-acetylation pathways for gangliosides. They also highlight the limitation of immuno-detection for the complete identification of
O
-acetylated ganglioside profiles in cancer cells.
...
PMID:Profiling of
O
-acetylated Gangliosides Expressed in Neuroectoderm Derived Cells. 3193 67
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